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EHD4

ANTIBODY INFORMATION
 
 

Antibody HPA049890

  

Antibody HPA049986

  

Antibody HPA052729

  

Antibody HPA066751

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA049890   HPA049986   HPA052729   HPA066751  

Host species

 Rabbit   Rabbit   Rabbit   Rabbit  

Clonality

 pAb   pAb   pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

 EHD3 - ENSG00000013016 [100%]
EHD2 - ENSG00000024422 [80%]
EHD1 - ENSG00000110047 [92%]
   EHD3 - ENSG00000013016 [100%]
EHD2 - ENSG00000024422 [85%]
EHD1 - ENSG00000110047 [98%]
     EHD3 - ENSG00000013016 [100%]
EHD2 - ENSG00000024422 [83%]
EHD1 - ENSG00000110047 [96%]
  

Released in version

 12   12   12   16  

References

        

VALIDATION SUMMARY
 
 

Antibody HPA049890

  

Antibody HPA049986

  

Antibody HPA052729

  

Antibody HPA066751

  
Immunocytochemistry

      Approved  
Immunohistochemistry

Approved   Approved   Approved    
Western blot

Supported   Supported   Uncertain   Supported  
Protein array

Supported   Supported   Supported   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA049890

  

Antibody HPA049986

  

Antibody HPA052729

  

Antibody HPA066751

  

Standard validation

       Approved  

       

Figure description

       Immunofluorescent staining of human cell line BJ shows localization to plasma membrane.  

Antibody dilution

       1:38  

Literature conformity

       The subcellular location is partly supported by literature or no literature is available.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA049890

  

Antibody HPA049986

  

Antibody HPA052729

  

Antibody HPA066751

  

Standard validation

 Approved   Approved   Approved    

       

Figure description

 Immunohistochemical staining of human kidney shows strong positivity in glomeruli.   Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in cells in glomeruli.   Immunohistochemical staining of human testis shows strong cytoplasmic positivity in cells in seminiferus ducts.    

Expression

 RNA: detected in 37 tissues
Protein: detected in 24 cell types		   RNA: detected in 37 tissues
Protein: detected in 19 cell types		   RNA: detected in 37 tissues
Protein: detected in 19 cell types		    

Retrieval

 HIER pH6   HIER pH6   HIER pH6    

Antibody dilution

 1:100   1:400   1:15    

Literature conformity

 Partly consistent with gene/protein characterization data.   Partly consistent with gene/protein characterization data.   Partly consistent with gene/protein characterization data.    

RNA consistency

 Mainly consistent with RNA expression data.   Mainly not consistent with RNA expression data.   Mainly not consistent with RNA expression data.    

WESTERN BLOT
 
 

Antibody HPA049890

  

Antibody HPA049986

  

Antibody HPA052729

  

Antibody HPA066751

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		   Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		   Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

       

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 61.9, 61.2, 61.1, 60.9, 60.6, 42.7, 16.9, 16.6   61.9, 61.2, 61.1, 60.9, 60.6, 46.1, 42.7, 34.7   61.2, 61.1   61.9, 61.2, 61.1, 60.9, 60.6, 46.1, 42.7, 34.7, 18.6  

Antibody dilution

 1:80   1:160   1:150   1:130  

PROTEIN ARRAY
 
 

Antibody HPA049890

  

Antibody HPA049986

  

Antibody HPA052729

  

Antibody HPA066751

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

       

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:600   1:1300   1:1200   1:1500  

ANTIGEN INFORMATION
 
 

Antibody HPA049890

  

Antibody HPA049986

  

Antibody HPA052729

  

Antibody HPA066751

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 51   53   51   75  

Antigen sequence

 
KLYKSKLLPLEEHYRFHEFHSPALEDADFDNKPMVLLVGQYSTGKTTFIR
Y
  
PDNRKLFEAEEQDLFRDIQSLPRNAALRKLNDLIKRARLAKVHAYIISSL
KKE
  
FGKENKKRELISRLPEIYIQLQREYQISAGDFPEVKAMQEQLENYDFTKF
H
  
FVCAQLPNPVLESISVIDTPGILSGEKQRISRGYDFAAVLEWFAERVDRI
ILLFDAHKLDISDEFSEVIKALKNH
 

Matching transcripts

 EHD4-001 - ENSP00000220325 [86%]
EHD4-201 - ENSP00000478504 [86%]
  EHD4-001 - ENSP00000220325 [87%]
EHD4-201 - ENSP00000478504 [87%]
  EHD4-001 - ENSP00000220325 [100%]
EHD4-201 - ENSP00000478504 [100%]
  EHD4-001 - ENSP00000220325 [83%]
EHD4-201 - ENSP00000478504 [83%]
 

Other gene match

 EHD3 - ENSG00000013016 [100%]
EHD2 - ENSG00000024422 [80%]
EHD1 - ENSG00000110047 [92%]
   EHD3 - ENSG00000013016 [100%]
EHD2 - ENSG00000024422 [85%]
EHD1 - ENSG00000110047 [98%]
     EHD3 - ENSG00000013016 [100%]
EHD2 - ENSG00000024422 [83%]
EHD1 - ENSG00000110047 [96%]
  
ANTIGEN VIEW
EHD4-001
EHD4-201
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.