EHD1 - Antibodies - The Human Protein Atlas

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EHD1

TISSUE

CELL

CANCER

ANTIBODY INFORMATION

Antibody HPA049890

Antibody HPA049986

Antibody HPA066751

Antibody HPA067747

Provider

Atlas Antibodies
Sigma-Aldrich

Product name

HPA049890

HPA049986

HPA066751

HPA067747

Host species

Rabbit

Clonality

pAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Other gene match

EHD3 - ENSG00000013016 [100%]
EHD2 - ENSG00000024422 [80%]
EHD4 - ENSG00000103966 [86%]

EHD3 - ENSG00000013016 [100%]
EHD2 - ENSG00000024422 [85%]
EHD4 - ENSG00000103966 [87%]

EHD3 - ENSG00000013016 [100%]
EHD2 - ENSG00000024422 [83%]
EHD4 - ENSG00000103966 [83%]

Released in version

References

VALIDATION SUMMARY

Antibody HPA049890

Antibody HPA049986

Antibody HPA066751

Antibody HPA067747

Immunocytochemistry

Immunohistochemistry

Western blot

Protein array

IMMUNOCYTOCHEMISTRY

Antibody HPA049890

Antibody HPA049986

Antibody HPA066751

Antibody HPA067747

Standard validation

Approved

Supported

Figure description

Immunofluorescent staining of human cell line BJ shows localization to plasma membrane.

Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane.

Antibody dilution

1:38

1:60

Literature conformity

The subcellular location is partly supported by literature or no literature is available.

The subcellular location is supported by literature.

IMMUNOHISTOCHEMISTRY

Antibody HPA049890

Antibody HPA049986

Antibody HPA066751

Antibody HPA067747

Standard validation

Approved

Figure description

Immunohistochemical staining of human kidney shows strong positivity in glomeruli.

Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in cells in glomeruli.

Expression

RNA: detected in 37 tissues
Protein: detected in 24 cell types

RNA: detected in 37 tissues
Protein: detected in 19 cell types

Retrieval

HIER pH6

Antibody dilution

1:100

1:400

Literature conformity

Partly consistent with gene/protein characterization data.

RNA consistency

Mainly not consistent with RNA expression data.

WESTERN BLOT

Antibody HPA049890

Antibody HPA049986

Antibody HPA066751

Antibody HPA067747

Standard validation

Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).

Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.

Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).

Figure description

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Target mass (kDa)

61.9, 61.2, 61.1, 60.9, 60.6, 42.7, 16.9, 16.6

61.9, 61.2, 61.1, 60.9, 60.6, 46.1, 42.7, 34.7

61.9, 61.2, 61.1, 60.9, 60.6, 46.1, 42.7, 34.7, 18.6

61.9, 42.7

Antibody dilution

1:80

1:160

1:130

1:200

PROTEIN ARRAY

Antibody HPA049890

Antibody HPA049986

Antibody HPA066751

Antibody HPA067747

Standard validation

Supported

Pass with single peak corresponding to interaction only with its own antigen.

Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:600

1:1300

1:1500

1:2400

ANTIGEN INFORMATION

Antibody HPA049890

Antibody HPA049986

Antibody HPA066751

Antibody HPA067747

Antigen

Recombinant protein fragment

Length (aa)

Antigen sequence

KLYKSKLLPLEEHYRFHEFHSPALEDADFDNKPMVLLVGQYSTGKTTFIR
Y

PDNRKLFEAEEQDLFRDIQSLPRNAALRKLNDLIKRARLAKVHAYIISSL
KKE

FVCAQLPNPVLESISVIDTPGILSGEKQRISRGYDFAAVLEWFAERVDRI
ILLFDAHKLDISDEFSEVIKALKNH

MEQPGTAASPVSGSMFSWVSKDARRKKEPELFQTVAEGLRQLYAQKLLP

Matching transcripts

EHD1-001 - ENSP00000320516 [92%]
EHD1-002 - ENSP00000352354 [92%]
EHD1-007 - ENSP00000404944 [92%]
EHD1-015 - ENSP00000403830 [92%]
EHD1-016 - ENSP00000399022 [92%]
EHD1-201 - ENSP00000479153 [92%]

EHD1-001 - ENSP00000320516 [98%]
EHD1-002 - ENSP00000352354 [98%]
EHD1-007 - ENSP00000404944 [98%]
EHD1-008 - ENSP00000391429 [98%]
EHD1-201 - ENSP00000479153 [98%]

EHD1-001 - ENSP00000320516 [96%]
EHD1-002 - ENSP00000352354 [96%]
EHD1-007 - ENSP00000404944 [96%]
EHD1-201 - ENSP00000479153 [96%]
EHD1-008 - ENSP00000391429 [95%]
EHD1-013 - ENSP00000396273 [95%]

EHD1-007 - ENSP00000404944 [100%]
EHD1-201 - ENSP00000479153 [100%]

Other gene match

EHD3 - ENSG00000013016 [100%]
EHD2 - ENSG00000024422 [80%]
EHD4 - ENSG00000103966 [86%]

EHD3 - ENSG00000013016 [100%]
EHD2 - ENSG00000024422 [85%]
EHD4 - ENSG00000103966 [87%]

EHD3 - ENSG00000013016 [100%]
EHD2 - ENSG00000024422 [83%]
EHD4 - ENSG00000103966 [83%]

ANTIGEN VIEW

EHD1-001	EHD1-002	EHD1-007	EHD1-008	EHD1-013	EHD1-015	EHD1-016	EHD1-201

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.