EIF3J - Antibodies - The Human Protein Atlas

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EIF3J

TISSUE

CELL

CANCER

ANTIBODY INFORMATION

Antibody HPA050977

Provider

Atlas Antibodies
Sigma-Aldrich

Product name

HPA050977

Host species

Rabbit

Clonality

pAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Other gene match

Released in version

References

VALIDATION SUMMARY

Antibody HPA050977

Immunocytochemistry

Immunohistochemistry

Approved

Western blot

Protein array

Supported

IMMUNOCYTOCHEMISTRY

Antibody HPA050977

Formal validation: Genetic

Validated

Significant downregulation > 25% by one siRNA.

Relative fluorescence intensity (RFI)

Figure description

Cytoplasmic region of segmented cells in 10x-images

Antibody dilution

1:52

Standard validation

Approved

Figure description

Immunofluorescent staining of human cell line A-431 shows localization to cytosol.

Antibody dilution

1:52

Literature conformity

The subcellular location is partly supported by literature or no literature is available.

IMMUNOHISTOCHEMISTRY

Antibody HPA050977

Standard validation

Approved

Figure description

Immunohistochemical staining of human pancreas shows strong cytoplasmic positivity in exocrine glandular cells.

Expression

RNA: detected in 37 tissues
Protein: detected in 75 cell types

Retrieval

HIER pH6

Antibody dilution

1:1500

Literature conformity

Consistent with extensive gene/protein characterization data.

RNA consistency

Mainly consistent with RNA expression data.

WESTERN BLOT

Antibody HPA050977

Formal validation: Genetic

Validated

Downregulation visible in one of two siRNA lanes

Figure description

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: siRNA 1
Lane 3: siRNA 2
Lane 4: Scrambled

Target mass (kDa)

29.1, 23, 22.9

Loading control

Total protein image

Antibody dilution

1:115

Standard validation

Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).

Figure description

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Target mass (kDa)

29.1, 23, 22.9

Antibody dilution

1:260

PROTEIN ARRAY

Antibody HPA050977

Standard validation

Supported

Pass with single peak corresponding to interaction only with its own antigen.

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:2050

ANTIGEN INFORMATION

Antibody HPA050977

Antigen

Recombinant protein fragment

Length (aa)

Antigen sequence

DSDSWDADAFSVEDPVRKVGGGGTAGGDRWEGEDEDED

Matching transcripts

EIF3J-001 - ENSP00000261868 [100%]
EIF3J-002 - ENSP00000440221 [100%]
EIF3J-003 - ENSP00000414548 [100%]

Other gene match

ANTIGEN VIEW

EIF3J-001	EIF3J-002	EIF3J-003

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.