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Immunohistochemical staining of human testis shows strong cytoplasmic positivity in cells of seminiferus ducts.
Expression
RNA: detected in 37 tissues Protein: detected in 41 cell types
Retrieval
HIER pH6
Antibody dilution
1:225
Literature conformity
Partly consistent with gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA040701
Standard validation
Supported
Single band corresponding to the predicted size in kDa (+/-20%).
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY412275)
Target mass (kDa)
110.2
Antibody dilution
1:250
PROTEIN ARRAY
Antibody HPA040701
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody dilution
1:3000
ANTIGEN INFORMATION
Antibody HPA040701
Antigen
Recombinant protein fragment
Length (aa)
103
Antigen sequence
LYVNRNGQKVRPLARWKGQLVESVGWNKALGTESSTGPILVGTAQGHIFE
AELSASEGGLFGPAPDLYFRPLYVLNEEGGPAPVCSLEAERGPDGRSFVI
ATT
