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ANTIBODY INFORMATION
 
 

Antibody HPA023099

  

Antibody HPA056734

  

Antibody CAB002138

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Novocastra
  

Product name

 HPA023099   HPA056734   NCL-CALBINDIN  

Host species

 Rabbit   Rabbit   Mouse  

Clonality

 pAb   pAb   mAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Not known  

Other gene match

      

Released in version

 5   13   1  

References

      

VALIDATION SUMMARY
 
 

Antibody HPA023099

  

Antibody HPA056734

  

Antibody CAB002138

  
Immunocytochemistry

Approved      
Immunohistochemistry

Supported   Supported   Supported  
Western blot

Supported   Supported   Uncertain  
Protein array

Supported   Supported    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA023099

  

Antibody HPA056734

  

Antibody CAB002138

  

Standard validation

 Approved      

     

Figure description

 Immunofluorescent staining of human cell line U-251 MG shows localization to vesicles.      

Antibody dilution

 1:400      

Literature conformity

 The subcellular location is partly supported by literature or no literature is available.      

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA023099

  

Antibody HPA056734

  

Antibody CAB002138

  

Standard validation

 Supported   Supported   Supported  

     

Figure description

 Immunohistochemical staining of human cerebellum shows strong cytoplasmic and nuclear positivity in Purkinje cells.   Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in cells in tubules.   Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in subset of cells in tubules.  

Expression

 RNA: detected in 35 tissues
Protein: detected in 8 cell types		   RNA: detected in 35 tissues
Protein: detected in 4 cell types		   RNA: detected in 35 tissues
Protein: detected in 10 cell types		  

Retrieval

 HIER pH6   HIER pH6   HIER pH6  

Antibody dilution

 1:50000   1:1800   1:1000  

Literature conformity

 Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

 Consistent with RNA expression data.   Consistent with RNA expression data.   Consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA023099

  

Antibody HPA056734

  

Antibody CAB002138

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		  

     

Figure description

 Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 30   30, 23.6, 14, 12   30, 23.6, 14, 12, 0.4  

Antibody dilution

 1:250   1:280   1:500  

PROTEIN ARRAY
 
 

Antibody HPA023099

  

Antibody HPA056734

  

Antibody CAB002138

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:3000   1:3350    

ANTIGEN INFORMATION
 
 

Antibody HPA023099

  

Antibody HPA056734

  

Antibody CAB002138

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Not known  

Length (aa)

 90   48    

Antigen sequence

 
QSSLITASQFFEIWLHFDADGSGYLEGKELQNLIQELQQARKKAGLELSP
EMKTFVDQYGQRDDGKIGIVELAHVLPTEENFLLLFRCQQ
  
IETEELKNFLKDLLEKANKTVDDTKLAEYTDLMLKLFDSNNDGKLELT
  

											 
																				

																		

										
											
Matching transcripts

												
																					CALB1-001 - ENSP00000265431 [100%] 

											 
																						CALB1-001 - ENSP00000265431 [100%] 
CALB1-007 - ENSP00000429246 [100%] 
CALB1-008 - ENSP00000429602 [100%] 
CALB1-010 - ENSP00000430281 [88%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
CALB1-001
CALB1-007
CALB1-008
CALB1-010
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
						

				 
			

		

			
	











	

		


			

				

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