Fields »
ABOUT
HELP
BLOG
ADAM2

ANTIBODY INFORMATION
 
 

Antibody HPA024621

  

Antibody HPA026581

  

Antibody CAB022390

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Chemicon
  

Product name

 HPA024621   HPA026581   MAB19292  

Host species

 Rabbit   Rabbit   Mouse  

Clonality

 pAb   pAb   mAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Not known  

Other gene match

      

Released in version

 5   5   5  

References

   1    

VALIDATION SUMMARY
 
 

Antibody HPA024621

  

Antibody HPA026581

  

Antibody CAB022390

  
Immunohistochemistry


Validated
   Supported   Supported  
Western blot

Uncertain   Uncertain   Uncertain  
Protein array

Supported   Supported    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA024621

  

Antibody HPA026581

  

Antibody CAB022390

  

Formal validation: Orthogonal
Validated

Spearman correlation >0.6 for protein and RNA expression in tissues.		      

 

 

      

Figure description

 Distribution of protein expression (antibody staining) and RNA expression (TPM) across 36 tissues.      
 

Standard validation

 Supported   Supported   Supported  

     

Figure description

 Immunohistochemical staining of human testis shows distinct cytoplasmic positivity in mature spermatogonia.   Immunohistochemical staining of human testis shows strong cytoplasmic positivity in spermatocytes.   Immunohistochemical staining of human testis shows strong cytoplasmic positivity in spermatids.  

Expression

 RNA: detected in 1 tissues
Protein: detected in 4 cell types		   RNA: detected in 1 tissues
Protein: detected in 10 cell types		   RNA: detected in 1 tissues
Protein: detected in 23 cell types		  

Retrieval

 HIER pH6   HIER pH6   HIER pH6  

Antibody dilution

 1:160   1:90   1:100  

Literature conformity

 Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.   Partly consistent with extensive gene/protein characterization data.  

RNA consistency

 Consistent with RNA expression data.   Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA024621

  

Antibody HPA026581

  

Antibody CAB022390

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		   Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		   Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		  

     

Figure description

      

Target mass (kDa)

 82.5, 80.2, 75.6, 64.8   82.5, 80.2, 75.6   82.5, 80.2, 75.6, 64.8  

Antibody dilution

 1:250   1:250   1:500  

PROTEIN ARRAY
 
 

Antibody HPA024621

  

Antibody HPA026581

  

Antibody CAB022390

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:3000   1:3000    

ANTIGEN INFORMATION
 
 

Antibody HPA024621

  

Antibody HPA026581

  

Antibody CAB022390

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Not known  

Length (aa)

 62   94    

Antigen sequence

 
CENCLFMSKERMCRPSFEECDLPEYCNGSSASCPENHYVQTGHPCGLNQW
ICIDGVCMSGDK
  
NVSYGIEPLESSVGFEHVIYQVKHKKADVSLYNEKDIESRDLSFKLQSVE
PQQDFAKYIEMHVIVEKQLYNHMGSDTTVVAQKVFQLIGLTNAI
   

Matching transcripts

 ADAM2-001 - ENSP00000265708 [100%]
ADAM2-002 - ENSP00000429352 [100%]
ADAM2-003 - ENSP00000343854 [100%]
ADAM2-004 - ENSP00000369182 [100%]
ADAM2-201 - ENSP00000483607 [100%]
  ADAM2-001 - ENSP00000265708 [100%]
ADAM2-002 - ENSP00000429352 [100%]
ADAM2-201 - ENSP00000483607 [100%]
ADAM2-003 - ENSP00000343854 [80%]
   

Other gene match

      
ANTIGEN VIEW
ADAM2-001
ADAM2-002
ADAM2-003
ADAM2-004
ADAM2-201

RELEVANT PUBLICATIONS
 
 

Antibody HPA024621

  

Antibody HPA026581

  

Antibody CAB022390

  

1.

   Lack of ADAM2, CALR3 and SAGE1 Cancer/Testis Antigen Expression in Lung and Breast Cancer
Maheswaran E et al
PLoS One 2015;10(8):e0134967
    
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.