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ASAH1

ANTIBODY INFORMATION
 
 

Antibody HPA005468

  

Provider

 Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA005468  

Host species

 Rabbit  

Clonality

 pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

  

Released in version

 4  

References

 5  

VALIDATION SUMMARY
 
 

Antibody HPA005468

  
Immunohistochemistry


Validated
  
Western blot

Supported  
Protein array

Supported  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA005468

  

Formal validation: Orthogonal
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		  

 

 

  

Figure description

 Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.  
 

Standard validation

 Supported  

 

Figure description

 Immunohistochemical staining of human epididymis shows strong granular positivity in glandular cells.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 56 cell types		  

Retrieval

 HIER pH6  

Antibody dilution

 1:300  

Literature conformity

 Consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA005468

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

 

Figure description

 Lane 1: Marker [kDa] 220, 112, 84, 47, 32, 26, 16.8
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 46.5, 44.7, 44, 41.8  

Antibody dilution

 1:250  

PROTEIN ARRAY
 
 

Antibody HPA005468

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

 

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:3000  

ANTIGEN INFORMATION
 
 

Antibody HPA005468

  

Antigen

 Recombinant protein fragment  

Length (aa)

 124  

Antigen sequence

 
ENSTSYEEAKNLLTKTKILAPAYFILGGNQSGEGCVITRDRKESLDVYEL
DAKQGRWYVVQTNYDRWKHPFFLDDRRTPAKMCLNRTSQENISFETMYDV
LSTKPVLNKLTVYTTLIDVTKGQF
 

Matching transcripts

 ASAH1-001 - ENSP00000262097 [100%]
ASAH1-002 - ENSP00000371152 [100%]
ASAH1-003 - ENSP00000326970 [100%]
ASAH1-004 - ENSP00000427751 [100%]
 

Other gene match

  
ANTIGEN VIEW
ASAH1-001
ASAH1-002
ASAH1-003
ASAH1-004

RELEVANT PUBLICATIONS
 
 

Antibody HPA005468

  

1.

 The cAMP-responsive element binding protein (CREB) regulates the expression of acid ceramidase (ASAH1) in H295R human adrenocortical cells
Lucki N et al
Biochim Biophys Acta 2009;1791(8):706-13
  

2.

 Genistein stimulates MCF-7 breast cancer cell growth by inducing acid ceramidase (ASAH1) gene expression
Lucki NC et al
J Biol Chem 2011;286(22):19399-409
  

3.

 Acid ceramidase (ASAH1) is a global regulator of steroidogenic capacity and adrenocortical gene expression
Lucki NC et al
Mol Endocrinol 2012;26(2):228-43
  

4.

 Acid ceramidase (ASAH1) represses steroidogenic factor 1-dependent gene transcription in H295R human adrenocortical cells by binding to the receptor
Lucki NC et al
Mol Cell Biol 2012;32(21):4419-31
  

5.

 Silencing diacylglycerol kinase-theta expression reduces steroid hormone biosynthesis and cholesterol metabolism in human adrenocortical cells
Cai K et al
Biochim Biophys Acta 2014;1841(4):552-62
Application: WB 		 
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.