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Immunohistochemical staining of human lymph node shows strong cytoplasmic positivity in reaction center cells.
Immunohistochemical staining of human lymph node shows distinct cytoplasmic positivity in reaction center cells.
Expression
RNA: detected in 27 tissues Protein: detected in 11 cell types
RNA: detected in 27 tissues Protein: detected in 30 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:250
1:25
Literature conformity
Consistent with extensive gene/protein characterization data.
Partly consistent with extensive gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA007419
Antibody CAB007754
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Weak band of predicted size but with additional bands of higher intensity also present.
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Figure description
Lane 1: Marker [kDa] 220, 112, 84, 47, 32, 26, 16.8 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
91.3, 90.4, 90
91.3, 90.4, 90, 57, 44.1
Antibody dilution
1:250
1:500
PROTEIN ARRAY
Antibody HPA007419
Antibody CAB007754
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.