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ANTIBODY INFORMATION
 
 

Antibody HPA038925

  

Antibody HPA038934

  

Antibody CAB000042

  

Antibody CAB010884

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   DakoCytomation
   SIGMA
  

Product name

 HPA038925   HPA038934   A0231   C7659  

Host species

 Rabbit   Rabbit   Rabbit   Mouse  

Clonality

 pAb   pAb   pAb   mAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Protein A/G   Not known  

Other gene match

 CGB2 - ENSG00000104818 [100%]
LHB - ENSG00000104826 [88%]
CGB5 - ENSG00000189052 [100%]
CGB7 - ENSG00000196337 [100%]
CGB8 - ENSG00000213030 [100%]
CTB-60B18.6 - ENSG00000267335 [100%]
CGB1 - ENSG00000267631 [100%]
   CGB2 - ENSG00000104818 [97%]
LHB - ENSG00000104826 [80%]
CGB5 - ENSG00000189052 [100%]
CGB7 - ENSG00000196337 [97%]
CGB8 - ENSG00000213030 [100%]
CGB1 - ENSG00000267631 [100%]
      

Released in version

 10   10   1   3  

References

        

VALIDATION SUMMARY
 
 

Antibody HPA038925

  

Antibody HPA038934

  

Antibody CAB000042

  

Antibody CAB010884

  
Immunocytochemistry

Approved   Approved      
Immunohistochemistry

Supported   Supported  

Validated
   Supported  
Western blot

Uncertain   Uncertain   Uncertain   Uncertain  
Protein array

Supported   Approved      

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA038925

  

Antibody HPA038934

  

Antibody CAB000042

  

Antibody CAB010884

  

Standard validation

 Approved   Approved      

       

Figure description

 Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.   Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.      

Antibody dilution

 1:179   1:200      

Literature conformity

 The subcellular location is partly supported by literature or no literature is available.   The subcellular location is partly supported by literature or no literature is available.      

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA038925

  

Antibody HPA038934

  

Antibody CAB000042

  

Antibody CAB010884

  

Formal validation: Orthogonal

    
Validated

Spearman correlation >0.6 for protein and RNA expression in tissues.		    

   
 

 

    

Figure description

     Distribution of protein expression (antibody staining) and RNA expression (TPM) across 37 tissues.    
 

Standard validation

 Supported   Supported   Supported   Supported  

       

Figure description

 Immunohistochemical staining of human placenta shows strong cytoplasmic positivity in trophoblastic cells.   Immunohistochemical staining of human placenta shows strong cytoplasmic positivity in trophoblastic cells.   Immunohistochemical staining of human placenta shows strong cytoplasmic positivity in trophoblastic cells.   Immunohistochemical staining of human placenta shows strong cytoplasmic positivity in trophoblastic cells.  

Expression

 RNA: detected in 1 tissues
Protein: detected in 24 cell types		   RNA: detected in 1 tissues
Protein: detected in 1 cell types		   RNA: detected in 1 tissues
Protein: detected in 1 cell types		   RNA: detected in 1 tissues
Protein: detected in 11 cell types		  

Retrieval

 HIER pH6   HIER pH6   Proteinase K.   HIER pH6  

Antibody dilution

 1:1200   1:2000   1:10000   1:100  

Literature conformity

 Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Consistent with RNA expression data.   Consistent with RNA expression data.   Consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA038925

  

Antibody HPA038934

  

Antibody CAB000042

  

Antibody CAB010884

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		   Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		   Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		   Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		  

       

Figure description

       Lane 1: Marker [kDa] 219, 111, 83, 48, 32, 26, 17
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 17.8, 17.7, 17.4, 16.7, 15.3, 14   17.8, 17.7, 17.4, 16.7   17.7   17.7  

Antibody dilution

 1:250   1:250   1:500   1:500  

PROTEIN ARRAY
 
 

Antibody HPA038925

  

Antibody HPA038934

  

Antibody CAB000042

  

Antibody CAB010884

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		      

       

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.      

Antibody dilution

 1:20000   1:6000      

ANTIGEN INFORMATION
 
 

Antibody HPA038925

  

Antibody HPA038934

  

Antibody CAB000042

  

Antibody CAB010884

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Native protein   Not known  

Length (aa)

 32   30      

Antigen sequence

 
PTMTRVLQGVLPALPQVVCNYRDVRFESIRLP
  
DHPLTCDDPRFQDSSSSKAPPPSLPSPSRL
  

											 
																						

											 
																				

																		

										
											
Matching transcripts

												
																					CGB-001 - ENSP00000349954 [100%] 

											 
																						CGB-001 - ENSP00000349954 [100%] 

											 
																						
											 
																						
											 
																				

																		

										
											
Other gene match

												
																					CGB2 - ENSG00000104818 [100%]
LHB - ENSG00000104826 [88%]
CGB5 - ENSG00000189052 [100%]
CGB7 - ENSG00000196337 [100%]
CGB8 - ENSG00000213030 [100%]
CTB-60B18.6 - ENSG00000267335 [100%]
CGB1 - ENSG00000267631 [100%]

											 
																						CGB2 - ENSG00000104818 [97%]
LHB - ENSG00000104826 [80%]
CGB5 - ENSG00000189052 [100%]
CGB7 - ENSG00000196337 [97%]
CGB8 - ENSG00000213030 [100%]
CGB1 - ENSG00000267631 [100%]

											 
																						
											 
																						
											 
																				

																		

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