TNNT1 - Antibodies - The Human Protein Atlas

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TNNT1

TISSUE

CELL

CANCER

ANTIBODY INFORMATION

Antibody HPA058448

Antibody CAB015168

Provider

Atlas Antibodies
Sigma-Aldrich

Santa Cruz Biotechnology

Product name

HPA058448

sc-28269

Host species

Rabbit

Clonality

pAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Protein A/G

Other gene match

Released in version

References

VALIDATION SUMMARY

Antibody HPA058448

Antibody CAB015168

Immunocytochemistry

Immunohistochemistry

Western blot

Protein array

IMMUNOCYTOCHEMISTRY

Antibody HPA058448

Antibody CAB015168

Standard validation

Approved

Figure description

Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm, cytosol & the Golgi apparatus.

Antibody dilution

1:25

Literature conformity

The subcellular location is partly supported by literature or no literature is available.

IMMUNOHISTOCHEMISTRY

Antibody HPA058448

Antibody CAB015168

Standard validation

Supported

Figure description

Immunohistochemical staining of human skeletal muscle shows strong cytoplasmic positivity in myocytes.

Expression

RNA: detected in 24 tissues
Protein: detected in 3 cell types

Retrieval

HIER pH6

Antibody dilution

1:900

Literature conformity

Consistent with extensive gene/protein characterization data.

RNA consistency

Consistent with RNA expression data.

WESTERN BLOT

Antibody HPA058448

Antibody CAB015168

Standard validation

Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).

Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.

Figure description

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Target mass (kDa)

32.9, 31.2, 24.4

32.9, 31.2, 30.1, 25.2, 24.4, 23.1, 19.2, 18.2, 17.4, 17.3, 11.9

Antibody dilution

1:330

1:500

PROTEIN ARRAY

Antibody HPA058448

Antibody CAB015168

Standard validation

Supported

Pass with single peak corresponding to interaction only with its own antigen.

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:3900

ANTIGEN INFORMATION

Antibody HPA058448

Antibody CAB015168

Antigen

Recombinant protein fragment

Recombinant protein

Length (aa)

Antigen sequence

EEEEAPEEPEPVAEPEEERPKPSRPVVPPLIPPKIPEGE

Matching transcripts

TNNT1-002 - ENSP00000467176 [100%]
TNNT1-003 - ENSP00000291901 [100%]
TNNT1-009 - ENSP00000470777 [100%]

Other gene match

ANTIGEN VIEW

TNNT1-002	TNNT1-003	TNNT1-009

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.