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SLC1A6

ANTIBODY INFORMATION
 
 

Antibody HPA041505

  

Antibody HPA044066

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA041505   HPA044066  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 11   11  

References

    

VALIDATION SUMMARY
 
 

Antibody HPA041505

  

Antibody HPA044066

  
Immunocytochemistry


Validated
  

Validated
  
Immunohistochemistry

Supported   Supported  
Western blot

Uncertain   Uncertain  
Protein array

Approved   Approved  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA041505

  

Antibody HPA044066

  

Formal validation: Independent
Validated

Antibody staining overlaps with antibody HPA044066.		  
Validated

Antibody staining overlaps with antibody HPA041505.		  
 

Standard validation

 Supported   Supported  

   

Figure description

 Immunofluorescent staining of human cell line A-431 shows localization to intermediate filaments.   Immunofluorescent staining of human cell line U-2 OS shows localization to intermediate filaments.  

Antibody dilution

 1:200   1:141  

Literature conformity

 The subcellular location is supported by literature.   The subcellular location is supported by literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA041505

  

Antibody HPA044066

  

Standard validation

 Supported   Supported  

   

Figure description

 Immunohistochemical staining of human cerebellum shows moderate positivity.   Immunohistochemical staining of human cerebellum shows moderate cytoplasmic positivity in Purkinje cells.  

Expression

 RNA: detected in 5 tissues
Protein: detected in 7 cell types		   RNA: detected in 5 tissues
Protein: detected in 42 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:2500   1:1200  

Literature conformity

 Partly consistent with gene/protein characterization data.   Partly consistent with gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA041505

  

Antibody HPA044066

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		   Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		  

   

Figure description

    

Target mass (kDa)

 61.6, 53.2, 53.1, 34.8   61.6, 53.2, 34.8, 18.1, 12.8, 8.6  

Antibody dilution

 1:250   1:250  

PROTEIN ARRAY
 
 

Antibody HPA041505

  

Antibody HPA044066

  

Standard validation

 Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		   Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:6000   1:12000  

ANTIGEN INFORMATION
 
 

Antibody HPA041505

  

Antibody HPA044066

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 67   56  

Antigen sequence

 
KQFKTQYSTRVVTRTMVRTENGSEPGASMPPPFSVENGTSFLENVTRALG
TLQEMLSFEETVPVPGS
  
MSSHGNSLFLRESGQRLGRVGWLQRLQESLQQRALRTRLRLQTMTLEHVL
RFLRRN
 

Matching transcripts

 SLC1A6-001 - ENSP00000409386 [100%]
SLC1A6-002 - ENSP00000221742 [100%]
SLC1A6-003 - ENSP00000471781 [100%]
SLC1A6-004 - ENSP00000446175 [100%]
SLC1A6-005 - ENSP00000471038 [100%]
  SLC1A6-002 - ENSP00000221742 [100%]
SLC1A6-003 - ENSP00000471781 [100%]
SLC1A6-004 - ENSP00000446175 [100%]
SLC1A6-005 - ENSP00000471038 [100%]
SLC1A6-008 - ENSP00000469551 [100%]
SLC1A6-010 - ENSP00000471129 [100%]
SLC1A6-011 - ENSP00000471562 [100%]
 

Other gene match

    
ANTIGEN VIEW
SLC1A6-001
SLC1A6-002
SLC1A6-003
SLC1A6-004
SLC1A6-005
SLC1A6-008
SLC1A6-010
SLC1A6-011
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.