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ANTIBODY INFORMATION
 
 

Antibody HPA024305

  

Antibody HPA052171

  

Antibody CAB018655

  

Antibody CAB040563

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Santa Cruz Biotechnology
   NCI-CPTC
  

Product name

 HPA024305   HPA052171   sc-33777   CPTC-GPI-1  

Host species

 Rabbit   Rabbit   Rabbit   Mouse  

Clonality

 pAb   pAb   pAb   mAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Protein A/G   Protein A/G  

Other gene match

        

Released in version

 5   16   5   7  

References

 1        

VALIDATION SUMMARY
 
 

Antibody HPA024305

  

Antibody HPA052171

  

Antibody CAB018655

  

Antibody CAB040563

  
Immunocytochemistry

Supported       Supported  
Immunohistochemistry


Validated
   Supported   Supported   Supported  
Western blot

Supported   Supported   Supported   Supported  
Protein array

Supported   Supported      

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA024305

  

Antibody HPA052171

  

Antibody CAB018655

  

Antibody CAB040563

  

Standard validation

 Supported       Supported  

       

Figure description

 Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm, plasma membrane & cytosol.       Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane & cytosol.  

Antibody dilution

 1:10       1:400  

Literature conformity

 The subcellular location is supported by literature.       The subcellular location is supported by literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA024305

  

Antibody HPA052171

  

Antibody CAB018655

  

Antibody CAB040563

  

Formal validation: Orthogonal
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		        

 

 

        

Figure description

 Distribution of protein expression (antibody staining) and RNA expression (TPM) across 43 cell lines.        
 

Standard validation

 Supported   Supported   Supported   Supported  

       

Figure description

 Immunohistochemical staining of human testis shows moderate cytoplasmic positivity in cells in seminiferous duct and Leydig cells.   Immunohistochemical staining of human testis shows strong cytoplasmic positivity in cells in seminiferous ducts..   Immunohistochemical staining of human testis shows strong cytoplasmic positivity in cells in seminiferous ducts.   Immunohistochemical staining of human testis shows strong cytoplasmic positivity in cells in seminiferous ducts nad Leydig cells.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 44 cell types		   RNA: detected in 37 tissues
Protein: detected in 78 cell types		   RNA: detected in 37 tissues
Protein: detected in 61 cell types		   RNA: detected in 37 tissues
Protein: detected in 69 cell types		  

Retrieval

 HIER pH6   HIER pH6   HIER pH6   HIER pH6  

Antibody dilution

 1:200   1:400   1:125   1:50000  

Literature conformity

 Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.   Partly consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA024305

  

Antibody HPA052171

  

Antibody CAB018655

  

Antibody CAB040563

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		   Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

       

Figure description

 Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 64.8, 64.3, 63.1, 53.4   64.8, 64.3, 63.1, 53.4   64.8, 64.3, 63.1, 53.4, 19.2, 18.7, 18.6, 18.3, 16.8, 15.4, 12.7   64.8, 64.3, 63.1, 53.4, 19.2, 18.7, 18.6, 18.3, 16.8, 15.4, 12.7  

Antibody dilution

 1:250   1:230   1:500   1:250  

PROTEIN ARRAY
 
 

Antibody HPA024305

  

Antibody HPA052171

  

Antibody CAB018655

  

Antibody CAB040563

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		      

       

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.      

Antibody dilution

 1:500   1:500      

ANTIGEN INFORMATION
 
 

Antibody HPA024305

  

Antibody HPA052171

  

Antibody CAB018655

  

Antibody CAB040563

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Recombinant protein   Recombinant protein  

Length (aa)

 90   97      

Antigen sequence

 
LGIWYINCFGCETHAMLPYDQYLHRFAAYFQQGDMESNGKYITKSGTRVD
HQTGPIVWGEPGTNGQHAFYQLIHQGTKMIPCDFLIPVQT
  
VINIGIGGSDLGPLMVTEALKPYSSGGPRVWYVSNIDGTHIAKTLAQLNP
ESSLFIIASKTFTTQETITNAETAKEWFLQAAKDPSAVAKHFVALST
  

											 
																						

											 
																				

																		

										
											
Matching transcripts

												
																					GPI-001 - ENSP00000405573 [100%] 
GPI-002 - ENSP00000465858 [100%] 
GPI-006 - ENSP00000348877 [100%] 
GPI-012 - ENSP00000467670 [100%] 

											 
																						GPI-002 - ENSP00000465858 [100%] 
GPI-006 - ENSP00000348877 [100%] 
GPI-012 - ENSP00000467670 [100%] 
GPI-001 - ENSP00000405573 [89%] 

											 
																						
											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																						
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
GPI-001
GPI-002
GPI-006
GPI-012
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
											

									
										
										
										
										
RELEVANT PUBLICATIONS

											
								

								

									 
								

								

									 
																			
											
											
											
Antibody HPA024305

												
										 
																				
											
											
											
Antibody HPA052171

												
										 
																				
											
											
											
Antibody CAB018655

												
										 
																				
											
											
											
Antibody CAB040563

												
										 
																		

																	

										
											
1.

												
																						Candidate serological biomarkers for cancer identified from the secretomes of 23 cancer cell lines and the human protein atlas

	Wu CC et al

	Mol Cell Proteomics 2010;9(6):1100-17

			
											 
																						
											 
																						
											 
																						
											 
																				

															
				
						

				 
			

		

			
	











	

		


			

				

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