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Immunofluorescent staining of human cell line PC-3 shows localization to nucleus & plasma membrane.
Antibody dilution
1:7
Literature conformity
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA035832
Antibody HPA064807
Antibody CAB011442
Standard validation
Approved
Approved
Figure description
Immunohistochemical staining of human lymph node shows membranous and cytoplasmic positivity in germinal center cells and non-germinal center cells.
Immunohistochemical staining of human tonsil shows cytoplasmic positivity in germinal center cells and non-germinal center cells.
Expression
RNA: detected in 34 tissues Protein: detected in 54 cell types
RNA: detected in 34 tissues Protein: detected in 64 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:150
1:150
Literature conformity
Partly consistent with extensive gene/protein characterization data.
Partly consistent with extensive gene/protein characterization data.
RNA consistency
Mainly not consistent with RNA expression data.
Mainly not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA035832
Antibody HPA064807
Antibody CAB011442
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Only bands not corresponding to the predicted size.
Uncertain
Analysis performed using a standard panel of samples. Single band larger than predicted size in kDa (+20%) but partly supported by experimental and/or bioinformatic data.
Uncertain
Analysis performed using a standard panel of samples. Single band larger than predicted size in kDa (+20%) but partly supported by experimental and/or bioinformatic data.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 219, 111, 83, 48, 32, 26, 17 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
39.8, 25.3
39.8, 33.9, 25.3, 22.3
39.8, 33.9, 25.3, 22.3
Antibody dilution
1:250
1:80
1:500
PROTEIN ARRAY
Antibody HPA035832
Antibody HPA064807
Antibody CAB011442
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
