PRPF31 - Antibodies - The Human Protein Atlas

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PRPF31

TISSUE

CELL

CANCER

ANTIBODY INFORMATION

Antibody HPA041939

Antibody HPA061873

Provider

Atlas Antibodies
Sigma-Aldrich

Product name

HPA041939

HPA061873

Host species

Rabbit

Clonality

pAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Other gene match

Released in version

References

VALIDATION SUMMARY

Antibody HPA041939

Antibody HPA061873

Immunocytochemistry

Immunohistochemistry

Western blot

Protein array

IMMUNOCYTOCHEMISTRY

Antibody HPA041939

Antibody HPA061873

Standard validation

Supported

Figure description

Immunofluorescent staining of human cell line PC-3 shows localization to nucleus.

Antibody dilution

1:4

Literature conformity

The subcellular location is supported by literature.

IMMUNOHISTOCHEMISTRY

Antibody HPA041939

Antibody HPA061873

Standard validation

Supported

Figure description

Immunohistochemical staining of human pancreas shows strong nuclear positivity in exocrine glandular cells and islets of Langerhans.

Expression

RNA: detected in 37 tissues
Protein: detected in 76 cell types

Retrieval

HIER pH6

Antibody dilution

1:140

Literature conformity

Consistent with extensive gene/protein characterization data.

RNA consistency

Consistent with RNA expression data.

WESTERN BLOT

Antibody HPA041939

Antibody HPA061873

Formal validation: Genetic

Validated

Downregulation visible in both siRNA lanes

Figure description

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: siRNA 1
Lane 3: siRNA 2
Lane 4: Scrambled

Target mass (kDa)

55.5, 54.9, 54.8, 31.4, 30.6, 28.9

Loading control

Total protein image

Antibody dilution

1:239

Standard validation

Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.

Uncertain

Analysis performed using a standard panel of samples.
No bands detected.

Figure description

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Target mass (kDa)

55.5, 54.9, 54.8, 31.4, 30.6, 28.9

Antibody dilution

1:250

1:80

PROTEIN ARRAY

Antibody HPA041939

Antibody HPA061873

Standard validation

Supported

Pass with single peak corresponding to interaction only with its own antigen.

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:6000

1:500

ANTIGEN INFORMATION

Antibody HPA041939

Antibody HPA061873

Antigen

Recombinant protein fragment

Length (aa)

103

101

Antigen sequence

TNATIMVVSVTASTTQGQQLSEEELERLEEACDMALELNASKHRIYEYVE
SRMSFIAPNLSIIIGASTAAKIMGVAGGLTNLSKMPACNIMLLGAQRKTL
SGF

SGDSVKTIAKLWDSKMFAEIMMKIEEYISKQAKASEVMGPVEAAPEYRVI
VDANNLTVEIENELNIIHKFIRDKYSKRFPELESLVPNALDYIRTVKELG
N

Matching transcripts

PRPF31-001 - ENSP00000324122 [100%]
PRPF31-003 - ENSP00000375635 [100%]
PRPF31-005 - ENSP00000395894 [100%]
PRPF31-006 - ENSP00000408980 [100%]
PRPF31-201 - ENSP00000405166 [100%]
PRPF31-009 - ENSP00000395089 [99%]

PRPF31-001 - ENSP00000324122 [100%]
PRPF31-003 - ENSP00000375635 [100%]
PRPF31-005 - ENSP00000395894 [100%]
PRPF31-006 - ENSP00000408980 [100%]
PRPF31-009 - ENSP00000395089 [100%]
PRPF31-201 - ENSP00000405166 [100%]

Other gene match

ANTIGEN VIEW

PRPF31-001	PRPF31-003	PRPF31-005	PRPF31-006	PRPF31-009	PRPF31-201

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.