ARMC6 - Antibodies - The Human Protein Atlas

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ARMC6

TISSUE

CELL

CANCER

ANTIBODY INFORMATION

Antibody HPA041420

Antibody HPA041681

Provider

Atlas Antibodies
Sigma-Aldrich

Product name

HPA041420

HPA041681

Host species

Rabbit

Clonality

pAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Other gene match

Released in version

References

VALIDATION SUMMARY

Antibody HPA041420

Antibody HPA041681

Immunocytochemistry

Immunohistochemistry

Western blot

Supported

Protein array

Supported

Approved

IMMUNOCYTOCHEMISTRY

Antibody HPA041420

Antibody HPA041681

Formal validation: Independent

Validated

Antibody staining overlaps with antibody HPA041681.

Validated

Antibody staining overlaps with antibody HPA041420.

Standard validation

Supported

Figure description

Immunofluorescent staining of human cell line A-431 shows localization to cytosol.

Immunofluorescent staining of human cell line HEK 293 shows localization to cytosol.

Antibody dilution

1:196

1:64

Literature conformity

The subcellular location is supported by literature.

IMMUNOHISTOCHEMISTRY

Antibody HPA041420

Antibody HPA041681

Formal validation: Independent

Validated

Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.

Validated

Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.

Figure description

Distribution of protein expression (antibody staining).
Pearson correlation with HPA041681 across 46 cell lines.

Distribution of protein expression (antibody staining).
Pearson correlation with HPA041420 across 46 cell lines.

Standard validation

Supported

Figure description

Immunohistochemical staining of human oral mucosa shows strong cytoplasmic positivity in squamous epithelial cells.

Immunohistochemical staining of human pancreas shows strong cytoplasmic positivity in exocrine glandular cells.

Expression

RNA: detected in 37 tissues
Protein: detected in 46 cell types

RNA: detected in 37 tissues
Protein: detected in 59 cell types

Retrieval

HIER pH6

Antibody dilution

1:500

1:100

Literature conformity

No avaliable gene/protein characterization data.

RNA consistency

Mainly consistent with RNA expression data.

WESTERN BLOT

Antibody HPA041420

Antibody HPA041681

Standard validation

Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.

Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).

Figure description

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Target mass (kDa)

54.1, 51.5, 28.4, 28.2, 17.7, 17.5

54.1, 51.5, 44.1

Antibody dilution

1:250

PROTEIN ARRAY

Antibody HPA041420

Antibody HPA041681

Standard validation

Supported

Pass with single peak corresponding to interaction only with its own antigen.

Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:6000

1:3000

ANTIGEN INFORMATION

Antibody HPA041420

Antibody HPA041681

Antigen

Recombinant protein fragment

Length (aa)

Antigen sequence

LSNIVKTAPKVSADGSQEPTHDILQMLSDLQESVASSRPQEVSAYLTRFC
DQCKQDKACRFLAAQKGAYPIIFTAWKLATAGDQGLLLQSLNALSVL

ATKAFLDNPGILSELCGTLSRLAIRNEFCQEVVDLGGLSILVSLLADCND
HQMRDQSGVQELVKQVLSTLRAIAGNDDVKDAIVR

Matching transcripts

ARMC6-001 - ENSP00000444156 [100%]
ARMC6-002 - ENSP00000376147 [100%]
ARMC6-005 - ENSP00000269932 [100%]
ARMC6-006 - ENSP00000376148 [100%]
ARMC6-007 - ENSP00000441948 [100%]
ARMC6-011 - ENSP00000446037 [100%]
ARMC6-012 - ENSP00000439269 [100%]
ARMC6-015 - ENSP00000446209 [98%]

ARMC6-001 - ENSP00000444156 [100%]
ARMC6-002 - ENSP00000376147 [100%]
ARMC6-003 - ENSP00000444341 [100%]
ARMC6-005 - ENSP00000269932 [100%]
ARMC6-006 - ENSP00000376148 [100%]

Other gene match

ANTIGEN VIEW

ARMC6-001	ARMC6-002	ARMC6-003	ARMC6-005	ARMC6-006	ARMC6-007	ARMC6-011	ARMC6-012	ARMC6-015

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.