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TFPI2

ANTIBODY INFORMATION
 
 

Antibody HPA049158

  

Antibody CAB010142

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Santa Cruz Biotechnology
  

Product name

 HPA049158   sc-48380  

Host species

 Rabbit   Mouse  

Clonality

 pAb   mAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Protein A/G  

Other gene match

    

Released in version

 12   3  

References

    

VALIDATION SUMMARY
 
 

Antibody HPA049158

  

Antibody CAB010142

  
Immunohistochemistry

Supported  

Validated
  
Western blot

Uncertain   Uncertain  
Protein array

Approved    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA049158

  

Antibody CAB010142

  

Formal validation: Orthogonal

  
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		  

 
 

 

  

Figure description

   Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.  
 

Standard validation

 Supported   Supported  

   

Figure description

 Immunohistochemical staining of human placenta shows strong cytoplasmic positivity in trophoblastic cells.   Immunohistochemical staining of human placenta shows strong cytoplasmic positivity in trophoblastic cells.  

Expression

 RNA: detected in 32 tissues
Protein: detected in 8 cell types		   RNA: detected in 32 tissues
Protein: detected in 4 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:1500   1:150  

Literature conformity

 Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA049158

  

Antibody CAB010142

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		   Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		  

   

Figure description

   Lane 1: Marker [kDa] 219, 111, 83, 48, 32, 26, 17
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 26.9, 12   26.9, 12  

Antibody dilution

 1:730   1:500  

PROTEIN ARRAY
 
 

Antibody HPA049158

  

Antibody CAB010142

  

Standard validation

 Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		    

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:5800    

ANTIGEN INFORMATION
 
 

Antibody HPA049158

  

Antibody CAB010142

  

Antigen

 Recombinant protein fragment   Recombinant protein  

Length (aa)

 53    

Antigen sequence

 
GNANNFYTWEACDDACWRIEKVPKVCRLQVSVDDQCEGSTEKYFFNLSSM
TCE
  

											 
																				

																		

										
											
Matching transcripts

												
																					TFPI2-001 - ENSP00000222543 [100%] 
TFPI2-003 - ENSP00000416370 [83%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
TFPI2-001
TFPI2-003
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
						

				 
			

		

			
	











	

		


			

				

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