Fields »
ABOUT
HELP
BLOG
TFEC

ANTIBODY INFORMATION
 
 

Antibody HPA063577

  

Antibody CAB032260

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Santa Cruz Biotechnology
  

Product name

 HPA063577   sc-11013  

Host species

 Rabbit   Goat  

Clonality

 pAb   msAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity  

Other gene match

    

Released in version

 16   6  

References

    

VALIDATION SUMMARY
 
 

Antibody HPA063577

  

Antibody CAB032260

  
Immunocytochemistry

Supported    
Immunohistochemistry

  Approved  
Western blot

Uncertain   Uncertain  
Protein array

Supported    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA063577

  

Antibody CAB032260

  

Standard validation

 Supported    

   

Figure description

 Immunofluorescent staining of human cell line CACO-2 shows localization to nucleoplasm.    

Antibody dilution

 1:175    

Literature conformity

 The subcellular location is supported by literature.    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA063577

  

Antibody CAB032260

  

Standard validation

   Approved  

   

Figure description

   Immunohistochemical staining of human tonsil shows nuclear positivity in germinal center and non-germinal center cells.  

Expression

   RNA: detected in 30 tissues
Protein: detected in 62 cell types		  

Retrieval

   HIER pH6  

Antibody dilution

   1:25  

Literature conformity

   Partly consistent with extensive gene/protein characterization data.  

RNA consistency

   Mainly not consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA063577

  

Antibody CAB032260

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		   Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		  

   

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		    

Target mass (kDa)

 38.8, 35.5, 31.6   38.8, 36.4, 35.5, 31.6, 22.7  

Antibody dilution

 1:590   1:500  

PROTEIN ARRAY
 
 

Antibody HPA063577

  

Antibody CAB032260

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:7050    

ANTIGEN INFORMATION
 
 

Antibody HPA063577

  

Antibody CAB032260

  

Antigen

 Recombinant protein fragment   Synthetic peptide  

Length (aa)

 51    

Antigen sequence

 
AIAFSDPLSYFTDLSFSAALKEEQRLDGMLLDDTISPFGTDPLLSATSPA
V
   

Matching transcripts

 TFEC-001 - ENSP00000265440 [100%]
TFEC-007 - ENSP00000318676 [100%]
TFEC-201 - ENSP00000387650 [100%]
   

Other gene match

    
ANTIGEN VIEW
TFEC-001
TFEC-007
TFEC-201
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.