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Immunohistochemical staining of human cerebral cortex shows strong cytoplasmic and nuclear positivity in neuronal cells, subset of glial cells showed strong nuclear staining.
Immunohistochemical staining of human cerebral cortex shows strong cytoplasmic and nuclear positivity in neuronal cells.
Expression
RNA: detected in 4 tissues Protein: detected in 19 cell types
RNA: detected in 4 tissues Protein: detected in 19 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:600
1:2700
Literature conformity
Partly consistent with extensive gene/protein characterization data.
Partly consistent with extensive gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA063001
Antibody CAB022222
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Current setup is not applicable due to low RNA count.
Uncertain
Analysis performed using a standard panel of samples. Current setup is not applicable due to low RNA count.
Figure description
Target mass (kDa)
42.6
42.6, 39.5, 38, 20.7, 10.1
Antibody dilution
1:130
1:500
PROTEIN ARRAY
Antibody HPA063001
Antibody CAB022222
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.