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LARP4B

ANTIBODY INFORMATION
 
 

Antibody HPA036566

  

Antibody HPA042738

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA036566   HPA042738  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 7   12  

References

    

VALIDATION SUMMARY
 
 

Antibody HPA036566

  

Antibody HPA042738

  
Immunocytochemistry

Supported   Supported  
Immunohistochemistry

Approved    
Western blot

Supported   Supported  
Protein array

Supported   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA036566

  

Antibody HPA042738

  

Standard validation

 Supported   Supported  

   

Figure description

 Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol.   Immunofluorescent staining of human cell line A-431 shows localization to nucleoli & cytosol.  

Antibody dilution

 1:125   1:185  

Literature conformity

 The subcellular location is supported by literature.   The subcellular location is supported by literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA036566

  

Antibody HPA042738

  

Standard validation

 Approved    

   

Figure description

 Immunohistochemical staining of human esophagus shows strong cytoplasmic positivity in squamous epithelial cells.    

Expression

 RNA: detected in 37 tissues
Protein: detected in 67 cell types		    

Retrieval

 HIER pH6    

Antibody dilution

 1:250    

Literature conformity

 No avaliable gene/protein characterization data.    

RNA consistency

 Mainly not consistent with RNA expression data.    

WESTERN BLOT
 
 

Antibody HPA036566

  

Antibody HPA042738

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

   

Figure description

 Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 80.6, 36.5, 20.3   80.6, 36.5, 16.5  

Antibody dilution

 1:250   1:250  

PROTEIN ARRAY
 
 

Antibody HPA036566

  

Antibody HPA042738

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:3000   1:6000  

ANTIGEN INFORMATION
 
 

Antibody HPA036566

  

Antibody HPA042738

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 90   84  

Antigen sequence

 
FENRLSSLIIGPSKERTLSADASVNTLPVVVSREPSVPASCAVSATYERS
PSPAHLPDDPKVAEKQRETHSVDRLPSALTATACKSVQVN
  
LRKPSYAEICQRTSKEPPSSPLQPQKEQKPNTVGCGKEEKKLAEPAERYR
EPPALKSTPGAPRDQRRPAGGRPSPSAMGKRLSR
 

Matching transcripts

 LARP4B-002 - ENSP00000326128 [100%]
LARP4B-007 - ENSP00000394545 [100%]
LARP4B-008 - ENSP00000403018 [100%]
LARP4B-201 - ENSP00000482767 [100%]
  LARP4B-002 - ENSP00000326128 [100%]
LARP4B-007 - ENSP00000394545 [100%]
LARP4B-011 - ENSP00000483973 [100%]
LARP4B-201 - ENSP00000482767 [100%]
 

Other gene match

    
ANTIGEN VIEW
LARP4B-002
LARP4B-007
LARP4B-008
LARP4B-011
LARP4B-201
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.