We use cookies to enhance the usability of our website. If you continue, we'll assume that you are happy to receive all cookies. More information. Don't show this again.
Antibody staining overlaps with GFP tagged protein
Validated
Antibody staining overlaps with GFP tagged protein
Figure description
Immunofluorescent staining of transgenic HeLa cells show antibody staining in nuclear membrane, cytoplasm & nucleus but excluded from the nucleoli and GFP expression in cytoplasm & nucleus but excluded from the nucleoli.
Immunofluorescent staining of transgenic HeLa cells show antibody staining in nuclear membrane, cytoplasm & nucleus but excluded from the nucleoli and GFP expression in cytoplasm & nucleus but excluded from the nucleoli.
Antibody dilution
1:9
1:24
Formal validation: Independent
Validated
Antibody staining overlaps with antibody HPA050302.
Validated
Antibody staining overlaps with antibody HPA029878.
Standard validation
Supported
Supported
Figure description
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm, nuclear membrane & cytosol.
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm, nuclear membrane & cytosol.
Antibody dilution
1:10
1:24
Literature conformity
The subcellular location is supported by literature.
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA029878
Antibody HPA050302
Antibody CAB034449
Standard validation
Supported
Supported
Figure description
Immunohistochemical staining of human testis shows strong nuclear and cytoplasmic positivity in cells in seminiferus ducts.
Immunohistochemical staining of human testis shows strong nuclear and cytoplasmic positivity in cells in seminiferus ducts.
Expression
RNA: detected in 37 tissues Protein: detected in 68 cell types
RNA: detected in 37 tissues Protein: detected in 77 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:8
1:16000
Literature conformity
Consistent with extensive gene/protein characterization data.
Consistent with extensive gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA029878
Antibody HPA050302
Antibody CAB034449
Standard validation
Supported
Analysis performed using a standard panel of samples. Single band corresponding to the predicted size in kDa (+/-20%).
Uncertain
Analysis performed using a standard panel of samples. No bands detected.
Supported
Analysis performed using a standard panel of samples. Single band corresponding to the predicted size in kDa (+/-20%).
Figure description
Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
97.2, 81.2, 76.4
97.2, 81.2, 76.4
97.2, 81.2, 76.4, 27.4, 16.5, 6.7
Antibody dilution
1:250
1:120
1:500
PROTEIN ARRAY
Antibody HPA029878
Antibody HPA050302
Antibody CAB034449
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
