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Immunohistochemical staining of human tonsil shows strong cytoplasmic positivity in non-germinal center cells, germinal center cells were moderately stained.
Immunohistochemical staining of human lymph node shows strong cytoplasmic positivity in germinal and non germinal center cells.
Expression
RNA: detected in 37 tissues Protein: detected in 22 cell types
RNA: detected in 37 tissues Protein: detected in 12 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:100
1:2000
Literature conformity
Consistent with extensive gene/protein characterization data.
Consistent with extensive gene/protein characterization data.
RNA consistency
Mainly not consistent with RNA expression data.
Mainly not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA043741
Antibody HPA061441
Antibody CAB046478
Standard validation
Supported
Analysis performed using a standard panel of samples. Single band corresponding to the predicted size in kDa (+/-20%).
Supported
Analysis performed using a standard panel of samples. Single band corresponding to the predicted size in kDa (+/-20%).
Supported
Analysis performed using a standard panel of samples. Band of predicted size in kDa (+/-20%) with additional bands present.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
43.7, 43.3
43.7, 43.3
43.7, 43.3, 41.6
Antibody dilution
1:250
1:760
1:500
PROTEIN ARRAY
Antibody HPA043741
Antibody HPA061441
Antibody CAB046478
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
