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ANTIBODY INFORMATION
 
 

Antibody HPA001352

  

Antibody HPA002549

  

Antibody CAB068250

  

Antibody CAB068251

  

Antibody CAB068252

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA001352   HPA002549   AMAb90767   AMAb90769   AMAb90768  

Host species

 Rabbit   Rabbit   Mouse   Mouse   Mouse  

Clonality

 pAb   pAb   mAb   mAb   mAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Protein A/G   Protein A/G   Protein A/G  

Other gene match

          

Released in version

 2   2   13   13   13  

References

 2   1        

VALIDATION SUMMARY
 
 

Antibody HPA001352

  

Antibody HPA002549

  

Antibody CAB068250

  

Antibody CAB068251

  

Antibody CAB068252

  
Immunocytochemistry

Approved          
Immunohistochemistry

Supported   Supported   Supported   Supported   Supported  
Western blot

Supported   Supported   Supported   Supported   Supported  
Protein array

Supported   Supported        

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA001352

  

Antibody HPA002549

  

Antibody CAB068250

  

Antibody CAB068251

  

Antibody CAB068252

  

Standard validation

 Approved          

         

Figure description

 Immunofluorescent staining of human cell line CACO-2 shows localization to vesicles.          

Antibody dilution

 1:58          

Literature conformity

 The subcellular location is partly supported by literature or no literature is available.          

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA001352

  

Antibody HPA002549

  

Antibody CAB068250

  

Antibody CAB068251

  

Antibody CAB068252

  

Standard validation

 Supported   Supported   Supported   Supported   Supported  

         

Figure description

 Immunohistochemical staining of human small intestine shows strong cytoplasmic positivity in glandular cells.   Immunohistochemical staining of human small intestine shows strong cytoplasmic positivity in glandular cells, plasma was distinctly stained.   Immunohistochemical staining of human small intestine shows strong cytoplasmic positivity in glandular cells, plasma was distinctly stained.   Immunohistochemical staining of human small intestine shows strong cytoplasmic positivity in glandular cells, plasma was distinctly stained.   Immunohistochemical staining of human small intestine shows strong cytoplasmic positivity in glandular cells.  

Expression

 RNA: detected in 8 tissues
Protein: detected in 11 cell types		   RNA: detected in 8 tissues
Protein: detected in 19 cell types		   RNA: detected in 8 tissues
Protein: detected in 20 cell types		   RNA: detected in 8 tissues
Protein: detected in 16 cell types		   RNA: detected in 8 tissues
Protein: detected in 19 cell types		  

Retrieval

 HIER pH6   HIER pH6   HIER pH6   HIER pH6   HIER pH6  

Antibody dilution

 1:2000   1:2000   1:4000   1:800   1:4000  

Literature conformity

 Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA001352

  

Antibody HPA002549

  

Antibody CAB068250

  

Antibody CAB068251

  

Antibody CAB068252

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

         

Figure description

 Lane 1: Marker [kDa] 206, 113, 82, 49, 32, 26, 17.8
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: A-431
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 206, 113, 82, 49, 32, 26, 17.8
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: A-431
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 45.4   45.4   45.4   45.4   45.4  

Antibody dilution

 1:250   1:250   1:1000   1:1000   1:1000  

PROTEIN ARRAY
 
 

Antibody HPA001352

  

Antibody HPA002549

  

Antibody CAB068250

  

Antibody CAB068251

  

Antibody CAB068252

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		        

         

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.        

Antibody dilution

 1:3000   1:3000        

ANTIGEN INFORMATION
 
 

Antibody HPA001352

  

Antibody HPA002549

  

Antibody CAB068250

  

Antibody CAB068251

  

Antibody CAB068252

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Recombinant protein   Recombinant protein   Recombinant protein  

Length (aa)

 134   148        

Antigen sequence

 
LEGLTFQMKKNAEELKARISASAEELRQRLAPLAEDVRGNLRGNTEGLQK
SLAELGGHLDQQVEEFRRRVEPYGENFNKALVQQMEQLRQKLGPHAGDVE
GHLSFLEKDLRDKVNSFFSTFKEKESQDKTLSLP
  
DKLGEVNTYAGDLQKKLVPFATELHERLAKDSEKLKEEIGKELEELRARL
LPHANEVSQKIGDNLRELQQRLEPYADQLRTQVNTQAEQLRRQLTPYAQR
MERVLRENADSLQASLRPHADELKAKIDQNVEELKGRLTPYADEFKVK
  

											 
																						

											 
																						

											 
																				

																		

										
											
Matching transcripts

												
																					APOA4-001 - ENSP00000350425 [100%] 

											 
																						APOA4-001 - ENSP00000350425 [99%] 

											 
																						
											 
																						
											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																						
											 
																						
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
APOA4-001
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
											

									
										
										
										
										
RELEVANT PUBLICATIONS

											
								

								

									 
								

								

									 
																			
											
											
											
Antibody HPA001352

												
										 
																				
											
											
											
Antibody HPA002549

												
										 
																				
											
											
											
Antibody CAB068250

												
										 
																				
											
											
											
Antibody CAB068251

												
										 
																				
											
											
											
Antibody CAB068252

												
										 
																		

																	

										
											
1.

												
																						Variance decomposition of protein profiles from antibody arrays using a longitudinal twin model

	Kato BS et al

	Proteome Sci 2011;9:73

			
											 
																							Variance decomposition of protein profiles from antibody arrays using a longitudinal twin model

	Kato BS et al

	Proteome Sci 2011;9:73

			
											 
																						
											 
																						
											 
																						
											 
																				

																		

										
											
2.

												
																						Global proteomic profiling reveals altered proteomic signature in schizophrenia serum

	Levin Y et al

	Mol Psychiatry 2010;15(11):1088-100

			
											 
																						
											 
																						
											 
																						
											 
																						
											 
																				

															
				
						

				 
			

		

			
	











	

		


			

				

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