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Immunofluorescent staining of human cell line U-2 OS shows localization to centrosome.
Antibody dilution
1:101
Literature conformity
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA037605
Antibody HPA037606
Standard validation
Uncertain
Uncertain
Figure description
Immunohistochemical staining of human kidney shows cytoplasmic and membranous positivity in cells in tubules.
Immunohistochemical staining of human kidney shows cytoplasmic positivity in cells in tubules.
Expression
RNA: detected in 36 tissues Protein: detected in 42 cell types
RNA: detected in 36 tissues Protein: detected in 50 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:1000
1:200
Literature conformity
No avaliable gene/protein characterization data.
No avaliable gene/protein characterization data.
RNA consistency
Mainly not consistent with RNA expression data.
Mainly not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA037605
Antibody HPA037606
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Weak band of predicted size but with additional bands of higher intensity also present.
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Figure description
Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
164.3, 14.1, 13.3
164.3
Antibody dilution
1:250
1:250
PROTEIN ARRAY
Antibody HPA037605
Antibody HPA037606
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
