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DDX6

ANTIBODY INFORMATION
 
 

Antibody HPA024201

  

Antibody HPA026644

  

Antibody CAB004668

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Abnova
  

Product name

 HPA024201   HPA026644   H00001656-M01  

Host species

 Rabbit   Rabbit   Mouse  

Clonality

 pAb   pAb   mAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Protein A/G  

Other gene match

      

Released in version

 6   5   2  

References

      

VALIDATION SUMMARY
 
 

Antibody HPA024201

  

Antibody HPA026644

  

Antibody CAB004668

  
Immunocytochemistry

Supported      
Immunohistochemistry

Approved   Approved   Approved  
Western blot

Supported   Supported   Supported  
Protein array

Approved   Supported    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA024201

  

Antibody HPA026644

  

Antibody CAB004668

  

Standard validation

 Supported      

     

Figure description

 Immunofluorescent staining of human cell line A-431 shows localization to cytoplasmic bodies.      

Antibody dilution

 1:66      

Literature conformity

 The subcellular location is supported by literature.      

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA024201

  

Antibody HPA026644

  

Antibody CAB004668

  

Standard validation

 Approved   Approved   Approved  

     

Figure description

 Immunohistochemical staining of human tonsil shows strong cytoplasmic positivity in germinal and non-germinal center cells.   Immunohistochemical staining of human tonsil shows moderate cytoplasmic positivity in germinal center cells.   Immunohistochemical staining of human lymph node shows strong cytoplasmic positivity in reaction center cells.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 67 cell types		   RNA: detected in 37 tissues
Protein: detected in 56 cell types		   RNA: detected in 37 tissues
Protein: detected in 73 cell types		  

Retrieval

 HIER pH6   HIER pH6   HIER pH6  

Antibody dilution

 1:500   1:35   1:200  

Literature conformity

 Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA024201

  

Antibody HPA026644

  

Antibody CAB004668

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

     

Figure description

 Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 230, 110, 82, 49.3, 32.2, 25.5, 17.6
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: A-431
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 54.4, 20.4   54.4   54.4, 20.4  

Antibody dilution

 1:250   1:250   1:500  

PROTEIN ARRAY
 
 

Antibody HPA024201

  

Antibody HPA026644

  

Antibody CAB004668

  

Standard validation

 Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:3000   1:3000    

ANTIGEN INFORMATION
 
 

Antibody HPA024201

  

Antibody HPA026644

  

Antibody CAB004668

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Recombinant protein  

Length (aa)

 117   132    

Antigen sequence

 
ENPVIMGLSSQNGQLRGPVKPTGGPGGGGTQTQQQMNQLKNTNTINNGTQ
QQAQSMTTTIKPGDDWKKTLKLPPKDLRIKTSDVTSTKGNEFEDYCLKRE
LLMGIFEMGWEKPSPIQ
  
EESIPIALSGRDILARAKNGTGKSGAYLIPLLERLDLKKDNIQAMVIVPT
RELALQVSQICIQVSKHMGGAKVMATTGGTNLRDDIMRLDDTVHVVIATP
GRILDLIKKGVAKVDHVQMIVLDEADKLLSQD
  

											 
																				

																		

										
											
Matching transcripts

												
																					DDX6-001 - ENSP00000442266 [100%] 
DDX6-002 - ENSP00000433704 [100%] 
DDX6-201 - ENSP00000483902 [100%] 
DDX6-202 - ENSP00000478754 [100%] 

											 
																						DDX6-001 - ENSP00000442266 [100%] 
DDX6-002 - ENSP00000433704 [100%] 
DDX6-202 - ENSP00000478754 [100%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
DDX6-001
DDX6-002
DDX6-201
DDX6-202
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
						

				 
			

		

			
	











	

		


			

				

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