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Immunohistochemical staining of human cerebral cortex shows strong nuclear positivity in neuronal cells.
Immunohistochemical staining of human cerebral cortex shows strong nuclear positivity in a subset of neuronal cells.
Expression
RNA: detected in 4 tissues Protein: detected in 6 cell types
RNA: detected in 4 tissues Protein: detected in 59 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:5000
1:400
Literature conformity
Partly consistent with gene/protein characterization data.
Not consistent with gene/protein characterization data.
RNA consistency
Consistent with RNA expression data.
Not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA039747
Antibody HPA069769
Standard validation
Supported
Band of predicted size in kDa (+/-20%) with additional bands present.
Supported
Analysis performed using a standard panel of samples. Single band corresponding to the predicted size in kDa (+/-20%).
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY402741)
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
45.3
45.3, 44.2, 42.5, 40.5, 37.7
Antibody dilution
1:250
1:170
PROTEIN ARRAY
Antibody HPA039747
Antibody HPA069769
Standard validation
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
