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Immunohistochemical staining of human gall bladder shows strong cytoplasmic and membranous positivity in glandular cells.
Expression
RNA: detected in 29 tissues Protein: detected in 63 cell types
Retrieval
HIER pH6
Antibody dilution
1:20
Literature conformity
Partly consistent with extensive gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA012743
Standard validation
Uncertain
No bands detected.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY422000)
Target mass (kDa)
81.9, 78.2, 75.7, 59
Antibody dilution
1:125
PROTEIN ARRAY
Antibody HPA012743
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
