We use cookies to enhance the usability of our website. If you continue, we'll assume that you are happy to receive all cookies. More information. Don't show this again.
Immunohistochemical staining of human testis shows strong cytoplasmic positivity in Leydig cells.
Expression
RNA: detected in 37 tissues Protein: detected in 34 cell types
Retrieval
HIER pH6
Antibody dilution
1:1800
Literature conformity
Partly consistent with extensive gene/protein characterization data.
RNA consistency
Mainly not consistent with RNA expression data.
Standard validation
Supported
Figure description
Wide expression, synaptic, nuclear type of staining as well as soma and processes.
WESTERN BLOT
Antibody HPA039460
Antibody HPA052272
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Weak band of predicted size but with additional bands of higher intensity also present.
Uncertain
Analysis performed using a standard panel of samples. Single band larger than predicted size in kDa (+20%) but partly supported by experimental and/or bioinformatic data.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
56.2, 54, 8.9
56.2, 54, 8.9
Antibody dilution
1:250
1:520
PROTEIN ARRAY
Antibody HPA039460
Antibody HPA052272
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
