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Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.
Antibody dilution
1:66
Literature conformity
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA031817
Antibody HPA057338
Standard validation
Approved
Figure description
Immunohistochemical staining of human pancreas shows moderate nuclear positivity in exocrine glandular cells and additional cytoplasmic staining in islets of Langerhans.
Expression
RNA: detected in 37 tissues Protein: detected in 55 cell types
Retrieval
HIER pH6
Antibody dilution
1:8000
Literature conformity
Partly consistent with extensive gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA031817
Antibody HPA057338
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Only bands not corresponding to the predicted size.
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
28.5, 28.4, 28.2, 28.1, 25.6, 20.5
28.5, 28.4, 28.2, 28.1, 25.6, 20.5
Antibody dilution
1:250
1:590
PROTEIN ARRAY
Antibody HPA031817
Antibody HPA057338
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
