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ATN1

ANTIBODY INFORMATION
 
 

Antibody HPA031619

  

Provider

 Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA031619  

Host species

 Rabbit  

Clonality

 pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

  

Released in version

 6  

References

 2  

VALIDATION SUMMARY
 
 

Antibody HPA031619

  
Immunocytochemistry


Validated
  
Immunohistochemistry


Validated
  
Western blot

Uncertain  
Protein array

Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA031619

  

Formal validation: Genetic
Validated

Significant downregulation > 25% by one siRNA.		  

 

Relative fluorescence intensity (RFI)

  

Figure description

 Nuclear region of segmented cells in 10x-images  

Antibody dilution

 1:149  
 

Standard validation

 Supported  

 

Figure description

 Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.  

Antibody dilution

 1:149  

Literature conformity

 The subcellular location is supported by literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA031619

  

Formal validation: Orthogonal
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		  

 

 

  

Figure description

 Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.  
 

Standard validation

 Supported  

 

Figure description

 Immunohistochemical staining of human placenta shows strong nuclear positivity in trophoblastic cells.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 56 cell types		  

Retrieval

 HIER pH6  

Antibody dilution

 1:200  

Literature conformity

 Consistent with gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA031619

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		  

 

Figure description

  

Target mass (kDa)

 125.4  

Antibody dilution

 1:250  

PROTEIN ARRAY
 
 

Antibody HPA031619

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

 

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:3000  

ANTIGEN INFORMATION
 
 

Antibody HPA031619

  

Antigen

 Recombinant protein fragment  

Length (aa)

 146  

Antigen sequence

 
HPYAMSPSLGSLRPYPPGPAHLPPPHSQVSYSQAGPNGPPVSSSSNSSSS
TSQGSYPCSHPSPSQGPQGAPYPFPPVPTVTTSSATLSTVIATVASSPAG
YKTASPPGPPPYGKRAPSPGAYKTATPPGYKPGSPPSFRTGTPPGY
 

Matching transcripts

 ATN1-001 - ENSP00000379915 [100%]
ATN1-002 - ENSP00000349076 [100%]
 

Other gene match

  
ANTIGEN VIEW
ATN1-001
ATN1-002

RELEVANT PUBLICATIONS
 
 

Antibody HPA031619

  

1.

 Epigenetic regulation of Atrophin1 by lysine-specific demethylase 1 is required for cortical progenitor maintenance
Zhang F et al
Nat Commun 2014;5:5815
  

2.

 Epigenetic regulation of Atrophin1 by lysine-specific demethylase 1 is required for cortical progenitor maintenance
Zhang F et al
Nat Commun 2014;5:5815
  
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.