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Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoli & cytosol.
Antibody dilution
1:400
Literature conformity
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA029666
Antibody HPA029667
Antibody HPA029668
Antibody HPA029669
Formal validation: Independent
Validated
Spearman correlation >0.6 for protein expression in tissues using independent antibodies.
Validated
Spearman correlation >0.6 for protein expression in tissues using independent antibodies.
Figure description
Distribution of protein expression (antibody staining). Spearman correlation with HPA029668 across 74 cell types.
Distribution of protein expression (antibody staining). Spearman correlation with HPA029666 across 74 cell types.
Standard validation
Uncertain
Uncertain
Uncertain
Uncertain
Figure description
Immunohistochemical staining of human urinary bladder shows cytoplasmic and membranous positivity in urothelial cells.
Immunohistochemical staining of human urinary bladder shows cytoplasmic and membranous positivity in urothelial cells.
Immunohistochemical staining of human urinary bladder shows cytoplasmic and membranous positivity in urothelial cells.
Immunohistochemical staining of human urinary bladder shows cytoplasmic and membranous positivity in urothelial cells.
Expression
RNA: detected in 36 tissues Protein: detected in 51 cell types
RNA: detected in 36 tissues Protein: detected in 31 cell types
RNA: detected in 36 tissues Protein: detected in 40 cell types
RNA: detected in 36 tissues Protein: detected in 70 cell types
Retrieval
HIER pH6
HIER pH6
HIER pH6
HIER pH6
Antibody dilution
1:750
1:25
1:10
1:7
Literature conformity
Not consistent with gene/protein characterization data.
Not consistent with gene/protein characterization data.
Not consistent with gene/protein characterization data.
Not consistent with gene/protein characterization data.
RNA consistency
Mainly not consistent with RNA expression data.
Mainly not consistent with RNA expression data.
Mainly not consistent with RNA expression data.
Mainly not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA029666
Antibody HPA029667
Antibody HPA029668
Antibody HPA029669
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Target too small/large to be analyzed with the present setup.
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Uncertain
Analysis performed using a standard panel of samples. No bands detected.
Uncertain
Analysis performed using a standard panel of samples. Target too small/large to be analyzed with the present setup.
Figure description
Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
632.8
632.8, 133.2
632.8, 133.2
632.8
Antibody dilution
1:250
1:250
1:250
1:250
PROTEIN ARRAY
Antibody HPA029666
Antibody HPA029667
Antibody HPA029668
Antibody HPA029669
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
