ERBB2IP - Antibodies - The Human Protein Atlas

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ERBB2IP

TISSUE

CELL

CANCER

ANTIBODY INFORMATION

Antibody HPA048606

Antibody HPA059863

Provider

Atlas Antibodies
Sigma-Aldrich

Product name

HPA048606

HPA059863

Host species

Rabbit

Clonality

pAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Other gene match

Released in version

References

VALIDATION SUMMARY

Antibody HPA048606

Antibody HPA059863

Immunocytochemistry

Immunohistochemistry

Western blot

Protein array

IMMUNOCYTOCHEMISTRY

Antibody HPA048606

Antibody HPA059863

Standard validation

Supported

Figure description

Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane.

Immunofluorescent staining of human cell line U-251 MG shows localization to nuclear speckles, plasma membrane & cell junctions.

Antibody dilution

1:182

1:127

Literature conformity

The subcellular location is supported by literature.

IMMUNOHISTOCHEMISTRY

Antibody HPA048606

Antibody HPA059863

Standard validation

Approved

Figure description

Immunohistochemical staining of human breast shows strong cytoplasmic positivity in myoepithelial cells.

Expression

RNA: detected in 37 tissues
Protein: detected in 37 cell types

Retrieval

HIER pH6

Antibody dilution

1:300

Literature conformity

Consistent with gene/protein characterization data.

RNA consistency

Mainly consistent with RNA expression data.

WESTERN BLOT

Antibody HPA048606

Antibody HPA059863

Standard validation

Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.

Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.

Figure description

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Target mass (kDa)

159, 158.3, 153.9, 153.5, 151.3, 146.1

Antibody dilution

1:250

1:420

PROTEIN ARRAY

Antibody HPA048606

Antibody HPA059863

Standard validation

Supported

Pass with single peak corresponding to interaction only with its own antigen.

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:3000

1:5100

ANTIGEN INFORMATION

Antibody HPA048606

Antibody HPA059863

Antigen

Recombinant protein fragment

Length (aa)

Antigen sequence

WHSKQNPQIDHASFPPQLLPRSESTENQSYAKHSANMNFSNHNNVRANTA
YHLHQRLGPARHGEMWAISPNDRLIPAVTRSTIQR

SENLKHIVNHDDVFEESEELSSDEEMKMAEMRPPLIETSINQPKVVALSN
NKKDDTKETDSLSDEVTHNSNQNNSNCSSPS

Matching transcripts

ERBB2IP-001 - ENSP00000370330 [100%]
ERBB2IP-002 - ENSP00000284037 [100%]
ERBB2IP-003 - ENSP00000370325 [100%]
ERBB2IP-004 - ENSP00000426632 [100%]
ERBB2IP-006 - ENSP00000422766 [100%]
ERBB2IP-007 - ENSP00000422015 [100%]
ERBB2IP-201 - ENSP00000370322 [100%]

Other gene match

ANTIGEN VIEW

ERBB2IP-001	ERBB2IP-002	ERBB2IP-003	ERBB2IP-004	ERBB2IP-006	ERBB2IP-007	ERBB2IP-201

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.