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ARSB

ANTIBODY INFORMATION
 
 

Antibody HPA037770

  

Antibody HPA037771

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA037770   HPA037771  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 7   7  

References

    

VALIDATION SUMMARY
 
 

Antibody HPA037770

  

Antibody HPA037771

  
Immunohistochemistry

Supported   Supported  
Western blot

Uncertain   Supported  
Protein array

Supported   Supported  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA037770

  

Antibody HPA037771

  

Standard validation

 Supported   Supported  

   

Figure description

 Immunohistochemical staining of human liver shows strong cytoplasmic positivity in hepatocytes.   Immunohistochemical staining of human cerebral cortex shows strong granular cytoplasmic positivity in neuronal cells.  

Expression

 RNA: detected in 36 tissues
Protein: detected in 62 cell types		   RNA: detected in 36 tissues
Protein: detected in 63 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:50   1:50  

Literature conformity

 Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA037770

  

Antibody HPA037771

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		   Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		  

   

Figure description

 Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 59.7   59.7, 46  

Antibody dilution

 1:250   1:250  

PROTEIN ARRAY
 
 

Antibody HPA037770

  

Antibody HPA037771

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:3000   1:3000  

ANTIGEN INFORMATION
 
 

Antibody HPA037770

  

Antibody HPA037771

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 71   92  

Antigen sequence

 
YPGCGYWFPPPSQYNVSEIPSSDPPTKTLWLFDIDRDPEERHDLSREYPH
IVTKLLSRLQFYHKHSVPVYF
  
TRCALDFRDGEEVATGYKNMYSTNIFTKRAIALITNHPPEKPLFLYLALQ
SVHEPLQVPEEYLKPYDFIQDKNRHHYAGMVSLMDEAVGNVT
 

Matching transcripts

 ARSB-001 - ENSP00000264914 [100%]
  ARSB-001 - ENSP00000264914 [100%]
ARSB-002 - ENSP00000379455 [100%]
ARSB-006 - ENSP00000456339 [100%]
 

Other gene match

    
ANTIGEN VIEW
ARSB-001
ARSB-002
ARSB-006
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.