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BNIP1

ANTIBODY INFORMATION
 
 

Antibody HPA008009

  

Antibody HPA008149

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA008009   HPA008149  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 3   16  

References

    

VALIDATION SUMMARY
 
 

Antibody HPA008009

  

Antibody HPA008149

  
Immunocytochemistry

  Supported  
Immunohistochemistry

Supported    
Western blot

Uncertain   Supported  
Protein array

Supported   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA008009

  

Antibody HPA008149

  

Standard validation

   Supported  

   

Figure description

   Immunofluorescent staining of human cell line MCF7 shows localization to endoplasmic reticulum.  

Antibody dilution

   1:50  

Literature conformity

   The subcellular location is supported by literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA008009

  

Antibody HPA008149

  

Standard validation

 Supported    

   

Figure description

 Immunohistochemical staining of human liver shows strong cytoplasmic positivity in hepatocytes.    

Expression

 RNA: detected in 37 tissues
Protein: detected in 45 cell types		    

Retrieval

 HIER pH6    

Antibody dilution

 1:10    

Literature conformity

 Consistent with extensive gene/protein characterization data.    

RNA consistency

 Mainly consistent with RNA expression data.    

WESTERN BLOT
 
 

Antibody HPA008009

  

Antibody HPA008149

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

   

Figure description

   Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 26.1, 22.4   31.1, 26.1  

Antibody dilution

 1:250   1:250  

PROTEIN ARRAY
 
 

Antibody HPA008009

  

Antibody HPA008149

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:500   1:3000  

ANTIGEN INFORMATION
 
 

Antibody HPA008009

  

Antibody HPA008149

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 78   118  

Antigen sequence

 
APQDVHVRICNQEIVKFDLEVKALIQDIRDCSGPLSALTELNTKVKEKFQ
QLRHRIQDLEQLAKEQDKESEKQLLLQE
  
KQMLSNQASWRKANLTCKIAIDNLEKAELLQGGDLLRQRKTTKESLAQTS
STITESLMGISRMMAQQVQQSEEAMQSLVTSSRTILDANEEFKSMSGTIQ
LGRKLITKYNRRELTDKL
 

Matching transcripts

 BNIP1-001 - ENSP00000239215 [100%]
BNIP1-003 - ENSP00000377365 [100%]
  BNIP1-001 - ENSP00000239215 [100%]
BNIP1-002 - ENSP00000231668 [100%]
 

Other gene match

    
ANTIGEN VIEW
BNIP1-001
BNIP1-002
BNIP1-003
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.