We use cookies to enhance the usability of our website. If you continue, we'll assume that you are happy to receive all cookies. More information. Don't show this again.
Immunohistochemical staining of human kidney shows cytoplasmic positivity with a granular pattern and extra-cellular positivity in cells in tubules.
Immunohistochemical staining of human kidney shows distinct membranous positivity in cells in tubules.
Immunohistochemical staining of human kidney shows distinct cytoplasmic positivity in cells in tubules with distinct extracellular material.
Expression
RNA: detected in 3 tissues Protein: detected in 9 cell types
RNA: detected in 3 tissues Protein: detected in 8 cell types
RNA: detected in 3 tissues Protein: detected in 32 cell types
Retrieval
HIER pH6
HIER pH6
HIER pH6
Antibody dilution
1:500
1:100
1:200
Literature conformity
Partly consistent with extensive gene/protein characterization data.
Partly consistent with extensive gene/protein characterization data.
Partly consistent with extensive gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA001616
Antibody HPA001645
Antibody CAB009809
Standard validation
Supported
Analysis performed using a standard panel of samples. Single band corresponding to the predicted size in kDa (+/-20%).
Supported
Single band corresponding to the predicted size in kDa (+/-20%).
Supported
Analysis performed using a standard panel of samples. Band of predicted size in kDa (+/-20%) with additional bands present.
Figure description
Lane 1: Marker [kDa] 206, 113, 82, 49, 32, 26, 17.8 Lane 2: RT4 Lane 3: U-251 MG Lane 4: A-431 Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY400319)
Lane 1: Marker [kDa] 219, 111, 83, 48, 32, 26, 17 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
72, 47.9, 43.8
72, 47.9, 43.8
72, 47.9, 43.8
Antibody dilution
1:250
1:250
1:500
PROTEIN ARRAY
Antibody HPA001616
Antibody HPA001645
Antibody CAB009809
Standard validation
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
