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Immunohistochemical staining of human rectum shows distinct positivity in plasma.
Immunohistochemical staining of human rectum shows distinct positivity in plasma.
Expression
RNA: detected in 2 tissues Protein: detected in 29 cell types
RNA: detected in 2 tissues Protein: detected in 13 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:150
1:2500
Literature conformity
Consistent with extensive gene/protein characterization data.
Consistent with extensive gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
Not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA050269
Antibody HPA054598
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Single band larger than predicted size in kDa (+20%) but partly supported by experimental and/or bioinformatic data.
Uncertain
Analysis performed using a standard panel of samples. Weak band of predicted size but with additional bands of higher intensity also present.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
59.6
59.6
Antibody dilution
1:450
1:1500
PROTEIN ARRAY
Antibody HPA050269
Antibody HPA054598
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.