Fields »
ABOUT
HELP
BLOG
ATP6V1A

ANTIBODY INFORMATION
 
 

Antibody HPA035083

  

Antibody HPA035084

  

Antibody CAB006910

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Upstate
  

Product name

 HPA035083   HPA035084   05-382  

Host species

 Rabbit   Rabbit   Mouse  

Clonality

 pAb   pAb   mAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Protein A/G  

Other gene match

      

Released in version

 12   12   3  

References

      

VALIDATION SUMMARY
 
 

Antibody HPA035083

  

Antibody HPA035084

  

Antibody CAB006910

  
Immunocytochemistry

Approved   Approved    
Immunohistochemistry

    Approved  
Western blot

Supported   Supported   Uncertain  
Protein array

Supported   Supported    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA035083

  

Antibody HPA035084

  

Antibody CAB006910

  

Standard validation

 Approved   Approved    

     

Figure description

 Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & cytosol.   Immunofluorescent staining of human cell line U-2 OS shows localization to nucleus, cytosol & vesicles.    

Antibody dilution

 1:200   1:123    

Literature conformity

 The subcellular location is partly supported by literature or no literature is available.   The subcellular location is partly supported by literature or no literature is available.    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA035083

  

Antibody HPA035084

  

Antibody CAB006910

  

Standard validation

     Approved  

     

Figure description

     Immunohistochemical staining of human placenta shows strong cytoplasmic and nuclear positivity in trophoblastic cells.  

Expression

     RNA: detected in 37 tissues
Protein: detected in 71 cell types		  

Retrieval

     HIER pH6  

Antibody dilution

     1:200  

Literature conformity

     Consistent with gene/protein characterization data.  

RNA consistency

     Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA035083

  

Antibody HPA035084

  

Antibody CAB006910

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		  

     

Figure description

 Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		    

Target mass (kDa)

 68.3   68.3, 24.2, 20.2   68.3, 24.2, 20.2  

Antibody dilution

 1:250   1:250   1:500  

PROTEIN ARRAY
 
 

Antibody HPA035083

  

Antibody HPA035084

  

Antibody CAB006910

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:3000   1:3000    

ANTIGEN INFORMATION
 
 

Antibody HPA035083

  

Antibody HPA035084

  

Antibody CAB006910

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Native protein  

Length (aa)

 87   94    

Antigen sequence

 
AIPGAFGCGKTVISQSLSKYSNSDVIIYVGCGERGNEMSEVLRDFPELTM
EVDGKVESIMKRTALVANTSNMPVAAREASIYTGITL
  
YVHGVSGPVVTACDMAGAAMYELVRVGHSELVGEIIRLEGDMATIQVYEE
TSGVSVGDPVLRTGKPLSVELGPGIMGAIFDGIQRPLSDISSQT
  

											 
																				

																		

										
											
Matching transcripts

												
																					ATP6V1A-001 - ENSP00000273398 [100%] 

											 
																						ATP6V1A-001 - ENSP00000273398 [100%] 
ATP6V1A-005 - ENSP00000419294 [100%] 
ATP6V1A-006 - ENSP00000417545 [85%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
ATP6V1A-001
ATP6V1A-005
ATP6V1A-006
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
						

				 
			

		

			
	











	

		


			

				

					Contact
				

				
				

					
					
					
					
				

				
			


			

				

					The Project
				
			
				
			

			
			

			
				

					The Human Protein Atlas
				

				
			

			
			

			

						The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.