Fields »
ABOUT
HELP
BLOG
SCAP

ANTIBODY INFORMATION
 
 

Antibody HPA004713

  

Provider

 Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA004713  

Host species

 Rabbit  

Clonality

 pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

  

Released in version

 3  

References

  

VALIDATION SUMMARY
 
 

Antibody HPA004713

  
Immunohistochemistry

Approved  
Western blot

Uncertain  
Protein array

Approved  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA004713

  

Standard validation

 Approved  

 

Figure description

 Immunohistochemical staining of human adrenal gland shows strong cytoplasmic positivity in glandular cells.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 75 cell types		  

Retrieval

 HIER pH6  

Antibody dilution

 1:5  

Literature conformity

 Partly consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA004713

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		  

 

Figure description

  

Target mass (kDa)

 139.7, 97.9  

Antibody dilution

 1:250  

PROTEIN ARRAY
 
 

Antibody HPA004713

  

Standard validation

 Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		  

 

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:2000  

ANTIGEN INFORMATION
 
 

Antibody HPA004713

  

Antigen

 Recombinant protein fragment  

Length (aa)

 119  

Antigen sequence

 
EVWDAIEGVLCCSSEEVSSGITALVFLDKRIVAARLNGSLDFFSLETHTA
LSPLQFRGTPGRGSSPASPVYSSSDTVACHLTHTVPCAHQKPITALKAAA
GRLVTGSQDHTLRVFRLED
 

Matching transcripts

 SCAP-001 - ENSP00000265565 [100%]
SCAP-202 - ENSP00000438956 [100%]
 

Other gene match

  
ANTIGEN VIEW
SCAP-001
SCAP-202
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.