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ABCC5

ANTIBODY INFORMATION
 
 

Antibody HPA044067

  

Antibody HPA052295

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA044067   HPA052295  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 14   10  

References

    

VALIDATION SUMMARY
 
 

Antibody HPA044067

  

Antibody HPA052295

  
Immunocytochemistry

Approved    
Immunohistochemistry

  Uncertain  
Western blot

Uncertain   Uncertain  
Protein array

Approved   Approved  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA044067

  

Antibody HPA052295

  

Standard validation

 Approved    

   

Figure description

 Immunofluorescent staining of human cell line RT4 shows localization to nucleoplasm, plasma membrane & cell junctions.    

Antibody dilution

 1:55    

Literature conformity

 The subcellular location is partly supported by literature or no literature is available.    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA044067

  

Antibody HPA052295

  

Standard validation

   Uncertain  

   

Figure description

   Immunohistochemical staining of human kidney shows strong membranous positivity in cells in tubules.  

Expression

   RNA: detected in 37 tissues
Protein: detected in 20 cell types		  

Retrieval

   HIER pH6  

Antibody dilution

   1:100  

Literature conformity

   Partly consistent with extensive gene/protein characterization data.  

RNA consistency

   Mainly not consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA044067

  

Antibody HPA052295

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		   Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		  

   

Figure description

   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 160.7, 155.7, 26.2, 25.3, 23.7, 16.7   160.7, 155.7, 26.2, 25.3, 23.7, 16.7  

Antibody dilution

 1:250   1:180  

PROTEIN ARRAY
 
 

Antibody HPA044067

  

Antibody HPA052295

  

Standard validation

 Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		   Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:3000   1:500  

ANTIGEN INFORMATION
 
 

Antibody HPA044067

  

Antibody HPA052295

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 139   139  

Antigen sequence

 
SPGYRSVRERTSTSGTHRDREDSKFRRTRPLECQDALETAARAEGLSLDA
SMHSQLRILDEEHPKGKYHHGLSALKPIRTTSKHQHPVDNAGLFSCMTFS
WLSSLARVAHKKGELSMEDVWSLSKHESSDVNCRRLERL
  
SPGYRSVRERTSTSGTHRDREDSKFRRTRPLECQDALETAARAEGLSLDA
SMHSQLRILDEEHPKGKYHHGLSALKPIRTTSKHQHPVDNAGLFSCMTFS
WLSSLARVAHKKGELSMEDVWSLSKHESSDVNCRRLERL
 

Matching transcripts

 ABCC5-001 - ENSP00000333926 [100%]
ABCC5-004 - ENSP00000376358 [100%]
ABCC5-005 - ENSP00000371934 [100%]
ABCC5-006 - ENSP00000404809 [100%]
ABCC5-201 - ENSP00000265586 [100%]
ABCC5-010 - ENSP00000399726 [96%]
  ABCC5-001 - ENSP00000333926 [100%]
ABCC5-004 - ENSP00000376358 [100%]
ABCC5-005 - ENSP00000371934 [100%]
ABCC5-006 - ENSP00000404809 [100%]
ABCC5-201 - ENSP00000265586 [100%]
ABCC5-010 - ENSP00000399726 [96%]
 

Other gene match

    
ANTIGEN VIEW
ABCC5-001
ABCC5-004
ABCC5-005
ABCC5-006
ABCC5-010
ABCC5-201
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.