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Immunofluorescent staining of human cell line U-2 OS shows localization to vesicles.
Antibody dilution
1:66
Literature conformity
The subcellular location is not consistent with literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA066717
Antibody HPA069797
Standard validation
Approved
Figure description
Immunohistochemical staining of human cerebral cortex shows cytoplasmic positivity in axonal projections.
Expression
RNA: detected in 31 tissues Protein: detected in 6 cell types
Retrieval
HIER pH6
Antibody dilution
1:40
Literature conformity
Partly consistent with extensive gene/protein characterization data.
RNA consistency
Mainly not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA066717
Antibody HPA069797
Standard validation
Uncertain
Analysis performed using a standard panel of samples. No bands detected.
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
