TP53I3 - Antibodies - The Human Protein Atlas

We use cookies to enhance the usability of our website. If you continue, we'll assume that you are happy to receive all cookies. More information. Don't show this again.

TP53I3

TISSUE

CELL

CANCER

ANTIBODY INFORMATION

Antibody HPA022012

Antibody HPA028742

Antibody CAB017479

Provider

Atlas Antibodies
Sigma-Aldrich

Santa Cruz Biotechnology

Product name

HPA022012

HPA028742

sc-30068

Host species

Rabbit

Clonality

pAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Protein A/G

Other gene match

Released in version

References

VALIDATION SUMMARY

Antibody HPA022012

Antibody HPA028742

Antibody CAB017479

Immunocytochemistry

Approved

Immunohistochemistry

Western blot

Supported

Protein array

Supported

Approved

IMMUNOCYTOCHEMISTRY

Antibody HPA022012

Antibody HPA028742

Antibody CAB017479

Standard validation

Approved

Figure description

Immunofluorescent staining of human cell line U-251 MG shows localization to vesicles.

Antibody dilution

1:135

Literature conformity

The subcellular location is partly supported by literature or no literature is available.

IMMUNOHISTOCHEMISTRY

Antibody HPA022012

Antibody HPA028742

Antibody CAB017479

Formal validation: Orthogonal

Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.

Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.

Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.

Figure description

Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.

Formal validation: Orthogonal

Validated

Spearman correlation >0.6 for protein and RNA expression in tissues.

Figure description

Distribution of protein expression (antibody staining) and RNA expression (TPM) across 37 tissues.

Formal validation: Independent

Validated

Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.

Validated

Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.

Figure description

Distribution of protein expression (antibody staining).
Pearson correlation with HPA028742 across 46 cell lines.

Distribution of protein expression (antibody staining).
Pearson correlation with HPA022012 across 46 cell lines.

Formal validation: Independent

Validated

Spearman correlation >0.6 for protein expression in tissues using independent antibodies.

Validated

Spearman correlation >0.6 for protein expression in tissues using independent antibodies.

Figure description

Distribution of protein expression (antibody staining).
Spearman correlation with HPA028742 across 75 cell types.

Distribution of protein expression (antibody staining).
Spearman correlation with HPA022012 across 75 cell types.

Standard validation

Supported

Figure description

Immunohistochemical staining of human kidney shows strong positivity in tubular cells.

Immunohistochemical staining of human kidney shows strong cytoplasmic and nuclear positivity in cells of tubules.

Immunohistochemical staining of human kidney shows strong cytoplasmic and nuclear positivity in renal cells.

Expression

RNA: detected in 37 tissues
Protein: detected in 58 cell types

RNA: detected in 37 tissues
Protein: detected in 55 cell types

RNA: detected in 37 tissues
Protein: detected in 51 cell types

Retrieval

HIER pH6

Antibody dilution

1:300

1:250

1:85

Literature conformity

No avaliable gene/protein characterization data.

RNA consistency

Mainly consistent with RNA expression data.

WESTERN BLOT

Antibody HPA022012

Antibody HPA028742

Antibody CAB017479

Standard validation

Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).

Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.

Figure description

Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Target mass (kDa)

35.5, 25.4, 22.7

35.5

35.5, 25.4, 22.7

Antibody dilution

1:250

1:500

PROTEIN ARRAY

Antibody HPA022012

Antibody HPA028742

Antibody CAB017479

Standard validation

Supported

Pass with single peak corresponding to interaction only with its own antigen.

Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:3000

ANTIGEN INFORMATION

Antibody HPA022012

Antibody HPA028742

Antibody CAB017479

Antigen

Recombinant protein fragment

Recombinant protein

Length (aa)

111

Antigen sequence

MLAVHFDKPGGPENLYVKEVAKPSPGEGEVLLKVAASALNRADLMQRQGQ
YDPPPGASNILGLEASGHVAELGPGCQGHWKIGDTAMALLPGGGQAQYVT
VPEGLLMPIPE

GRWVLYGLMGGGDINGPLFSKLLFKRGSLITSLLRSRDNKYKQMLVNAFT
EQILPHFSTEGPQRLLPVLDRIYPVTEIQEAHKYMEANKNIGKIVLEL

Matching transcripts

TP53I3-001 - ENSP00000238721 [100%]
TP53I3-002 - ENSP00000337834 [100%]
TP53I3-005 - ENSP00000384414 [100%]
TP53I3-006 - ENSP00000389620 [95%]

TP53I3-001 - ENSP00000238721 [100%]
TP53I3-002 - ENSP00000337834 [100%]

Other gene match

ANTIGEN VIEW

TP53I3-001	TP53I3-002	TP53I3-005	TP53I3-006

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.