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GRB14

ANTIBODY INFORMATION
 
 

Antibody HPA035052

  

Antibody HPA035053

  

Antibody CAB022294

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Chemicon
  

Product name

 HPA035052   HPA035053   MAB1167  

Host species

 Rabbit   Rabbit   Mouse  

Clonality

 pAb   pAb   mAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Protein A/G  

Other gene match

      

Released in version

 12   13   5  

References

      

VALIDATION SUMMARY
 
 

Antibody HPA035052

  

Antibody HPA035053

  

Antibody CAB022294

  
Immunocytochemistry

Uncertain      
Immunohistochemistry

  Approved   Approved  
Western blot

Uncertain   Supported   Uncertain  
Protein array

Supported   Supported    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA035052

  

Antibody HPA035053

  

Antibody CAB022294

  

Standard validation

 Uncertain      

     

Figure description

 Immunofluorescent staining of human cell line A549 shows localization to nucleoplasm & cytosol.      

Antibody dilution

 1:5      

Literature conformity

 The subcellular location is not consistent with literature.      

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA035052

  

Antibody HPA035053

  

Antibody CAB022294

  

Standard validation

   Approved   Approved  

     

Figure description

   Immunohistochemical staining of human testis shows moderate cytoplasmic positivity in cells in seminiferus ducts, Leydig cells were strongly stained.   Immunohistochemical staining of human pancreas shows strong cytoplasmic positivity in exocrine cells.  

Expression

   RNA: detected in 9 tissues
Protein: detected in 56 cell types		   RNA: detected in 9 tissues
Protein: detected in 43 cell types		  

Retrieval

   HIER pH6   HIER pH6  

Antibody dilution

   1:55   1:15  

Literature conformity

   Partly consistent with extensive gene/protein characterization data.   Partly consistent with extensive gene/protein characterization data.  

RNA consistency

   Mainly not consistent with RNA expression data.   Mainly not consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA035052

  

Antibody HPA035053

  

Antibody CAB022294

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		   Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		   Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		  

     

Figure description

   Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 61, 47.6   61, 47.6, 18.3   61, 47.6, 18.3  

Antibody dilution

 1:250   1:250   1:500  

PROTEIN ARRAY
 
 

Antibody HPA035052

  

Antibody HPA035053

  

Antibody CAB022294

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:500   1:3000    

ANTIGEN INFORMATION
 
 

Antibody HPA035052

  

Antibody HPA035053

  

Antibody CAB022294

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Synthetic peptide  

Length (aa)

 70   90    

Antigen sequence

 
KEPRHLQFFSEFGNSDIYVSLAGKKKHGAPTNYGFCFKPNKAGGPRDLKM
LCAEEEQSRTCWVTAIRLLK
  
PELCCSPFTSVLSADLFPKANSRKKQVIKVYSEDETSRALDVPSDITARD
VCQLLILKNHYIDDHSWTLFEHLPHIGVERTIEDHELVIE
  

											 
																				

																		

										
											
Matching transcripts

												
																					GRB14-001 - ENSP00000263915 [100%] 
GRB14-002 - ENSP00000416786 [100%] 

											 
																						GRB14-001 - ENSP00000263915 [100%] 
GRB14-002 - ENSP00000416786 [100%] 
GRB14-005 - ENSP00000401702 [100%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
GRB14-001
GRB14-002
GRB14-005
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
						

				 
			

		

			
	











	

		


			

				

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