SPTBN1 - Antibodies - The Human Protein Atlas

We use cookies to enhance the usability of our website. If you continue, we'll assume that you are happy to receive all cookies. More information. Don't show this again.

SPTBN1

TISSUE

CELL

CANCER

ANTIBODY INFORMATION

Antibody HPA012685

Antibody HPA013149

Provider

Atlas Antibodies
Sigma-Aldrich

Product name

HPA012685

HPA013149

Host species

Rabbit

Clonality

pAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Other gene match

Released in version

References

VALIDATION SUMMARY

Antibody HPA012685

Antibody HPA013149

Immunocytochemistry

Approved

Immunohistochemistry

Western blot

Supported

Uncertain

Protein array

Supported

IMMUNOCYTOCHEMISTRY

Antibody HPA012685

Antibody HPA013149

Standard validation

Approved

Figure description

Immunofluorescent staining of human cell line A-431 shows localization to the Golgi apparatus.

Antibody dilution

1:11

Literature conformity

The subcellular location is partly supported by literature or no literature is available.

IMMUNOHISTOCHEMISTRY

Antibody HPA012685

Antibody HPA013149

Formal validation: Orthogonal

Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.

Figure description

Distribution of protein expression (antibody staining) and RNA expression (TPM) across 43 cell lines.

Formal validation: Independent

Validated

Spearman correlation >0.6 for protein expression in tissues using independent antibodies.

Validated

Spearman correlation >0.6 for protein expression in tissues using independent antibodies.

Figure description

Distribution of protein expression (antibody staining).
Spearman correlation with HPA013149 across 68 cell types.

Distribution of protein expression (antibody staining).
Spearman correlation with HPA012685 across 68 cell types.

Standard validation

Supported

Figure description

Immunohistochemical staining of human duodenum shows strong membranous positivity in glandular cells.

Immunohistochemical staining of human duodenum shows strong cytoplasmic and membranous positivity in glandular cells.

Expression

RNA: detected in 37 tissues
Protein: detected in 65 cell types

RNA: detected in 37 tissues
Protein: detected in 63 cell types

Retrieval

HIER pH6

Antibody dilution

1:75

1:400

Literature conformity

Consistent with extensive gene/protein characterization data.

RNA consistency

Consistent with RNA expression data.

WESTERN BLOT

Antibody HPA012685

Antibody HPA013149

Standard validation

Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.

Uncertain

Analysis performed using a standard panel of samples.
Target too small/large to be analyzed with the present setup.

Figure description

Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Target mass (kDa)

274.8, 274.6, 251.4

Antibody dilution

1:250

PROTEIN ARRAY

Antibody HPA012685

Antibody HPA013149

Standard validation

Supported

Pass with single peak corresponding to interaction only with its own antigen.

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:500

1:3000

ANTIGEN INFORMATION

Antibody HPA012685

Antibody HPA013149

Antigen

Recombinant protein fragment

Length (aa)

108

126

Antigen sequence

PLATSTDHGHNLQTVQLLIKKNQTLQKEIQGHQPRIDDIFERSQNIVTDS
SSLSAEAIRQRLADLKQLWGLLIEETEKRHRRLEEAHRAQQYYFDAAEAE
AWMSEQEL

SLLHQFQADADDIDAWMLDILKIVSSSDVGHDEYSTQSLVKKHKDVAEEI
ANYRPTLDTLHEQASALPQEHAESPDVRGRLSGIEERYKEVAELTRLRKQ
ALQDTLALYKMFSEADACELWIDEKE

Matching transcripts

SPTBN1-001 - ENSP00000349259 [100%]
SPTBN1-002 - ENSP00000334156 [100%]
SPTBN1-201 - ENSP00000479037 [100%]

Other gene match

ANTIGEN VIEW

SPTBN1-001	SPTBN1-002	SPTBN1-201

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.