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Immunofluorescent staining of human cell line U-2 OS shows localization to nuclear bodies & vesicles.
Antibody dilution
1:8
Literature conformity
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA036685
Antibody HPA036686
Antibody CAB033842
Standard validation
Supported
Supported
Supported
Figure description
Immunohistochemical staining of human duodenum shows strong cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in cells in tubules.
Immunohistochemical staining of human colon shows moderate cytoplasmic positivity in glandular cells.
Expression
RNA: detected in 37 tissues Protein: detected in 56 cell types
RNA: detected in 37 tissues Protein: detected in 71 cell types
RNA: detected in 37 tissues Protein: detected in 73 cell types
Retrieval
HIER pH6
HIER pH6
HIER pH6
Antibody dilution
1:15
1:500
1:75
Literature conformity
Partly consistent with gene/protein characterization data.
Partly consistent with gene/protein characterization data.
Partly consistent with gene/protein characterization data.
RNA consistency
Mainly not consistent with RNA expression data.
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA036685
Antibody HPA036686
Antibody CAB033842
Standard validation
Supported
Analysis performed using a standard panel of samples. Band of predicted size in kDa (+/-20%) with additional bands present.
Uncertain
No bands detected.
Uncertain
Analysis performed using a standard panel of samples. Weak band of predicted size but with additional bands of higher intensity also present.
Figure description
Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY413350)