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Immunofluorescent staining of human cell line U-251 MG shows localization to focal adhesion sites.
Antibody dilution
1:34
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA006185
Antibody CAB002424
Standard validation
Approved
Approved
Figure description
Immunohistochemical staining of human esophagus shows strong membranous positivity in squamous epithelial cells.
Immunohistochemical staining of human placenta shows moderate membranous positivity in trophoblasts.
Expression
RNA: detected in 34 tissues Protein: detected in 30 cell types
RNA: detected in 34 tissues Protein: detected in 6 cell types
Retrieval
HIER pH6
HIER pH9
Antibody dilution
1:75
1:50
Literature conformity
Partly consistent with extensive gene/protein characterization data.
Not consistent with gene/protein characterization data.
RNA consistency
Mainly not consistent with RNA expression data.
Not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA006185
Antibody CAB002424
Standard validation
Uncertain
Single band larger than predicted size in kDa (+20%) but partly supported by experimental and/or bioinformatic data.
Uncertain
Analysis performed using a standard panel of samples. Single band larger than predicted size in kDa (+20%) but partly supported by experimental and/or bioinformatic data.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY423561)
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
32.5
32.5, 22, 17.8
Antibody dilution
1:250
1:500
PROTEIN ARRAY
Antibody HPA006185
Antibody CAB002424
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
