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Immunohistochemical staining of human spleen shows strong cytoplasmic positivity in cells in red pulp and cells in white pulp.
Immunohistochemical staining of human tonsil shows moderate cytoplasmic positivity in reaction center cells and lymphoid cells outside reaction centra.
Expression
RNA: detected in 37 tissues Protein: detected in 14 cell types
RNA: detected in 37 tissues Protein: detected in 14 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:50
1:100
Literature conformity
Consistent with extensive gene/protein characterization data.
Consistent with extensive gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA003739
Antibody HPA070706
Antibody CAB009737
Standard validation
Uncertain
Analysis performed using a standard panel of samples. No bands detected.
Uncertain
Analysis performed using a standard panel of samples. Weak band of predicted size but with additional bands of higher intensity also present.
Uncertain
Analysis performed using a standard panel of samples. No bands detected.
Figure description
Lane 1: Marker [kDa] 230, 110, 82, 49.3, 32.2, 25.5, 17.6 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 219, 111, 83, 48, 32, 26, 17 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
52, 51.3, 50.4, 50.3
52, 51.3, 50.4, 50.3, 40.5
52, 51.3, 50.4, 50.3, 40.5, 15.1, 12.5, 9.3
Antibody dilution
1:250
1:340
1:500
PROTEIN ARRAY
Antibody HPA003739
Antibody HPA070706
Antibody CAB009737
Standard validation
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
