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Immunofluorescent staining of human cell line A-431 shows localization to nucleus, nuclear bodies & nuclear membrane.
Antibody dilution
1:45
Literature conformity
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA056956
Antibody CAB004269
Standard validation
Supported
Figure description
Immunohistochemical staining of human testis shows strong nuclear positivity in cells in seminiferus ducts.
Expression
RNA: detected in 37 tissues Protein: detected in 70 cell types
Retrieval
HIER pH6
Antibody dilution
1:200
Literature conformity
Consistent with extensive gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA056956
Antibody CAB004269
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Uncertain
Analysis performed using a standard panel of samples. Only bands not corresponding to the predicted size.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
16.6, 11.6, 8.8, 6.7
16.6, 11.6, 8.8, 7.2, 6.7
Antibody dilution
1:150
1:500
PROTEIN ARRAY
Antibody HPA056956
Antibody CAB004269
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
