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Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.
Antibody dilution
1:15
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA011216
Antibody CAB002222
Standard validation
Supported
Supported
Figure description
Immunohistochemical staining of human skin shows distinct cytoplasmic positivity in a subset of cells that resembled Langerhans cells.
Immunohistochemical staining of human skin shows distinct positivity in a subset of cells that resemble Langerhans cells.
Expression
RNA: detected in 20 tissues Protein: detected in 6 cell types
RNA: detected in 20 tissues Protein: detected in 6 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:25
1:150
Literature conformity
Consistent with extensive gene/protein characterization data.
Consistent with extensive gene/protein characterization data.
RNA consistency
Consistent with RNA expression data.
Consistent with RNA expression data.
WESTERN BLOT
Antibody HPA011216
Antibody CAB002222
Standard validation
Supported
Single band corresponding to the predicted size in kDa (+/-20%).
Uncertain
Analysis performed using a standard panel of samples. No bands detected.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY402461)
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
36.7
36.7
Antibody dilution
1:250
1:500
PROTEIN ARRAY
Antibody HPA011216
Antibody CAB002222
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
