AMPD2 - Antibodies - The Human Protein Atlas

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AMPD2

TISSUE

CELL

CANCER

ANTIBODY INFORMATION

Antibody HPA027137

Antibody HPA045760

Antibody HPA050590

Provider

Atlas Antibodies
Sigma-Aldrich

Product name

HPA027137

HPA045760

HPA050590

Host species

Rabbit

Clonality

pAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Other gene match

Released in version

References

VALIDATION SUMMARY

Antibody HPA027137

Antibody HPA045760

Antibody HPA050590

Immunocytochemistry

Immunohistochemistry

Western blot

Protein array

IMMUNOCYTOCHEMISTRY

Antibody HPA027137

Antibody HPA045760

Antibody HPA050590

Formal validation: Genetic

Validated

Significant downregulation > 25% by both siRNA:s.

Relative fluorescence intensity (RFI)

Figure description

Cytoplasmic region of segmented cells in 10x-images

Antibody dilution

1:77

Standard validation

Approved

Figure description

Immunofluorescent staining of human cell line MCF7 shows localization to cytosol.

Antibody dilution

1:77

Literature conformity

The subcellular location is partly supported by literature or no literature is available.

IMMUNOHISTOCHEMISTRY

Antibody HPA027137

Antibody HPA045760

Antibody HPA050590

Formal validation: Independent

Validated

Spearman correlation >0.6 for protein expression in tissues using independent antibodies.

Validated

Spearman correlation >0.6 for protein expression in tissues using independent antibodies.

Figure description

Distribution of protein expression (antibody staining).
Spearman correlation with HPA050590 across 73 cell types.

Distribution of protein expression (antibody staining).
Spearman correlation with HPA045760 across 73 cell types.

Standard validation

Approved

Figure description

Immunohistochemical staining of human cerebellum shows strong cytoplasmic and nuclear positivity in Purkinje cells.

Immunohistochemical staining of human cerebellum shows cytoplasmic positivity in Purkinje cells.

Immunohistochemical staining of human pancreas shows strong cytoplasmic positivity in islets of Langerhans.

Expression

RNA: detected in 37 tissues
Protein: detected in 28 cell types

RNA: detected in 37 tissues
Protein: detected in 44 cell types

RNA: detected in 37 tissues
Protein: detected in 33 cell types

Retrieval

HIER pH6

Antibody dilution

1:150

1:1000

1:10000

Literature conformity

Partly consistent with extensive gene/protein characterization data.

RNA consistency

Mainly not consistent with RNA expression data.

Standard validation

Approved

Figure description

Staining observed in subsets of neuronal cell bodies and synapses. Most abundant in circumventricular organs.

WESTERN BLOT

Antibody HPA027137

Antibody HPA045760

Antibody HPA050590

Formal validation: Genetic

Validated

Downregulation visible in both siRNA lanes

Figure description

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: siRNA 1
Lane 3: siRNA 2
Lane 4: Scrambled

Target mass (kDa)

100.7, 98.4, 92.9, 92.1, 88.3, 88.2, 21.8, 10.2

Loading control

Total protein image

Antibody dilution

1:170

Standard validation

Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.

Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.

Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.

Figure description

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Target mass (kDa)

100.7, 98.4, 92.9, 92.1, 88.3, 88.2

100.7, 98.4, 92.9, 92.1, 88.3, 88.2, 21.8, 10.2

100.7, 98.4

Antibody dilution

1:250

1:380

1:450

PROTEIN ARRAY

Antibody HPA027137

Antibody HPA045760

Antibody HPA050590

Standard validation

Supported

Pass with single peak corresponding to interaction only with its own antigen.

Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).

Supported

Pass with single peak corresponding to interaction only with its own antigen.

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:3000

1:3050

1:3600

ANTIGEN INFORMATION

Antibody HPA027137

Antibody HPA045760

Antibody HPA050590

Antigen

Recombinant protein fragment

Length (aa)

Antigen sequence

VLEREFQRVTISGEEKCGVPFTDLLDAAKSVVRALFIREKYMALSLQSFC
PTTRRYLQQLAEKPLETRTYEQGPDTPVSADAP

MDGKCKEIAEELFTRSLAESELRSAPYEFPEESPIEQLEERRQRLERQIS
QDVKLEPDILLRAKQDFLKTDSDSDLQLYKEQGE

RRKGLDVAEPGPSRCRSDSPAVAAVVPAMASYPSGSGKPKAKYPFKKRAS
LQASTAAPEARGGLGAPPLQSARSLPGPAPCLKHFPL

Matching transcripts

AMPD2-001 - ENSP00000345498 [100%]
AMPD2-002 - ENSP00000358855 [100%]
AMPD2-005 - ENSP00000436541 [100%]
AMPD2-006 - ENSP00000351573 [100%]
AMPD2-007 - ENSP00000437164 [100%]
AMPD2-011 - ENSP00000256578 [100%]
AMPD2-012 - ENSP00000377292 [100%]

AMPD2-001 - ENSP00000345498 [100%]
AMPD2-002 - ENSP00000358855 [100%]
AMPD2-005 - ENSP00000436541 [100%]
AMPD2-007 - ENSP00000437164 [100%]
AMPD2-011 - ENSP00000256578 [100%]
AMPD2-015 - ENSP00000431975 [100%]
AMPD2-018 - ENSP00000431904 [100%]
AMPD2-006 - ENSP00000351573 [88%]
AMPD2-012 - ENSP00000377292 [88%]

AMPD2-005 - ENSP00000436541 [100%]
AMPD2-011 - ENSP00000256578 [100%]
AMPD2-002 - ENSP00000358855 [95%]

Other gene match

ANTIGEN VIEW

AMPD2-001	AMPD2-002	AMPD2-005	AMPD2-006	AMPD2-007	AMPD2-011	AMPD2-012	AMPD2-015	AMPD2-018

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.