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Immunofluorescent staining of human cell line U-251 MG shows localization to vesicles.
Antibody dilution
1:67
Literature conformity
The subcellular location is not consistent with literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA012113
Antibody HPA016980
Standard validation
Approved
Approved
Figure description
Immunohistochemical staining of human placenta shows strong cytoplasmic positivity in decidual cells.
Immunohistochemical staining of human vulva/anal skin shows positivity in fibroblasts.
Expression
RNA: detected in 36 tissues Protein: detected in 63 cell types
RNA: detected in 36 tissues Protein: detected in 47 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:150
1:75
Literature conformity
Partly consistent with extensive gene/protein characterization data.
Partly consistent with extensive gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA012113
Antibody HPA016980
Standard validation
Supported
Analysis performed using a standard panel of samples. Band of predicted size in kDa (+/-20%) with additional bands present.
Supported
Band of predicted size in kDa (+/-20%) with additional bands present.
Figure description
Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY411682)
Target mass (kDa)
90.6, 83.4, 78.9
90.6, 83.4, 78.9
Antibody dilution
1:250
1:250
PROTEIN ARRAY
Antibody HPA012113
Antibody HPA016980
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
