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Immunofluorescent staining of human cell line PC-3 shows localization to nucleus.
Antibody dilution
1:109
Literature conformity
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA050785
Antibody HPA055308
Formal validation: Independent
Validated
Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.
Validated
Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.
Figure description
Distribution of protein expression (antibody staining). Pearson correlation with HPA055308 across 46 cell lines.
Distribution of protein expression (antibody staining). Pearson correlation with HPA050785 across 46 cell lines.
Formal validation: Independent
Validated
Spearman correlation >0.6 for protein expression in tissues using independent antibodies.
Validated
Spearman correlation >0.6 for protein expression in tissues using independent antibodies.
Figure description
Distribution of protein expression (antibody staining). Spearman correlation with HPA055308 across 72 cell types.
Distribution of protein expression (antibody staining). Spearman correlation with HPA050785 across 72 cell types.
Standard validation
Supported
Supported
Figure description
Immunohistochemical staining of human testis shows strong nuclear positivity in cells in seminiferus ducts.
Immunohistochemical staining of human testis shows strong nuclear positivity in cells in seminiferus ducts.
Expression
RNA: detected in 37 tissues Protein: detected in 75 cell types
RNA: detected in 37 tissues Protein: detected in 69 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:350
1:700
Literature conformity
Consistent with extensive gene/protein characterization data.
Consistent with extensive gene/protein characterization data.
RNA consistency
Consistent with RNA expression data.
Consistent with RNA expression data.
WESTERN BLOT
Antibody HPA050785
Antibody HPA055308
Standard validation
Supported
Band of predicted size in kDa (+/-20%) with additional bands present.
Supported
Single band corresponding to the predicted size in kDa (+/-20%).
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY419640)
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY419640)
Target mass (kDa)
19.4
19.4
Antibody dilution
1:125
1:250
PROTEIN ARRAY
Antibody HPA050785
Antibody HPA055308
Standard validation
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
